核农学报2016，Vol.30Issue(12)：2451-2459,9.DOI:10.11869/j.issn.100-8551.2016.12.2451
外源NO对Ca(NO3)2胁迫下番茄幼苗PSⅡ功能及光能分配利用的影响
Effect of Exogenous Nitric Oxide on PSH Function and Distribution and Utilization of Luminous Energy in Tomato Seedlings Under Stress of Ca(NO3)2
摘要
Abstract
To explore the photo-physiological mechanism for exogenous nitric oxide (NO) relieving the damage of secondary salinization on tomato seedling,a hydrophonic experiment was carried out with tomato seedlings of Qin Feng Bao Guan as test material in greenhouse to investigate the effect of NO donor sodium nitroprusside (SNP) on PS Ⅱ photochemical efficiency,distribution of excitation energy and absorption and utilization of luminous energy by antenna pigment within tomato seedlings under stress of Ca(NO3)2 of 80 mmol·L-1 by the technique of chlorophyll fluorescence kinetics.The results indicated that spraying SNP effectively relieved the Ca (NO3)2 stress on PS Ⅱ damage.In addition,the maximum photochemical efficiency (Fv/Fm),antenna conversion efficiency (F'v/F'm),practical photochemical efficiency (ΦPSⅡ),photochemical fluorescence quenching quotient (qP),quotient of absorbed light used in photochemical reaction (P) and non-photochemical dissipation used for reaction center (P680) (Ex) were significantly increased by 4.93％,39.31％,92.84％,39.00％,92.83％ and 10.33％,respectively.Moreover,the original fluorescence (Fo),non-photochemical fluorescence quenching quotient (NPQ),the relative deviation (β/α-1) from full balance between two photosystems and quotient of absorbed light used in antenna heat dissipation (D) were significantly reduced by 7.20％,13.22％,43.09％ and 24.70％,respectively,which was compared with the stress in 15d after the treatment.In conclusion,the results indicate that exogenous NO can alleviate photo-inhibition caused by Ca(NO3) 2 stress by improving the photochemical activity of PS Ⅱ and enhancing non-photochemical dissipation of the PS Ⅱ center,and enhance the salt-tolerance of tomato seedlings,which provide theoretical and technical basis on utilizing exogenous NO to remit secondary salinization barriers.关键词
一氧化氮/Ca(NO3)2胁迫/番茄幼苗/PSⅡ光化学效率/光能分配Key words
nitric oxide/Ca (NO3) 2 stress/tomato seedlings/PSⅡ photochemical efficiency/distribution of luminous energy引用本文复制引用
孙德智,韩晓日,彭靖,范富,张庆国..外源NO对Ca(NO3)2胁迫下番茄幼苗PSⅡ功能及光能分配利用的影响[J].核农学报,2016,30(12):2451-2459,9.基金项目
国家公益性行业(农业)科研专项(201303095-15),内蒙古自然科学基金项目(2013MS0605),内蒙古民族大学科学研究基金资助项目(NMDYB15097) （农业）
