中医药导报2016,Vol.22Issue(20):37-39,3.
广西不同产地瑶药猛老虎中白花丹醌的含量比较研究
Comparison Study on the Plumbagin Content in Plumbago Zeylanica Collected from Different Areas of Guangxi
摘要
Abstract
Objective:To compare plumbagin content in Plumbago zeylanica from different areas of Guangxi,in order to provide references for appropriate utilization and development of Plumbago zeylanica (A Yao medicine named Menglaohu) medicinal materials.Methods:The content of plumbagin from Plumbago zeylanica was determined by HPLC.The analysis was carried out on a ZORBAX Eclipse XDB C18 column (4.6 mm×250 mm,5 μm).The mobile phase was consisted of methanol:water (60∶40,V∶V) in isocratic elution with flow rate 1.0 mL/ rin and the detection wave length was 265 nm.Results:The plumbagin showed good linearity (r2=0.9999) in a relatively wide concentration range (0.104 μg~2.08 μg).The recovery rate of the method was in the range of 98.05%-100.52%,and the RSD was 1.12%.The plumbagin content range of 16 batches of Plumbago zeylanica from 12 places of Guangxi was 0.004%~0.311%.Conclusion:Plumbagin contents in Plumbago zeylanica from different areas of Guangxi had certain differences and the HPLC method can be used to control the quality of the Plumbago zeylanica medicinal materials.关键词
瑶药猛老虎/高效液相色谱法/白花丹醌Key words
Plumbago zeylanica Linn./High performance liquid chromatography/plumbagin分类
医药卫生引用本文复制引用
黎宁兰,龙海荣,唐春风,谷筱玉,陈乾平,彭玉德..广西不同产地瑶药猛老虎中白花丹醌的含量比较研究[J].中医药导报,2016,22(20):37-39,3.基金项目
国家自然科学基金地区基金项目(81560623) (81560623)
广西卫生厅中医药科技专项(GZZY13-35) (GZZY13-35)
广西医疗.卫生适宜技术研究与开发课题(S201416-01) (S201416-01)