江汉大学学报（自然科学版）2016，Vol.44Issue(5)：448-455,8.DOI:10.16389/j.cnki.cn42-1737/n.2016.05.012
星点设计-效应面法优化半枝莲总生物碱提取工艺
Optimization of Extraction Process of Scutellaria barbata Total Alkaloids with Central-Composite-Design Response-Surface Method
摘要
Abstract
Objective To optimize the extraction process of Scutellariabar bata total alkaloids with central-composite-design response-surface method. Methods Five main factors including ethanol volume fraction ,extraction time ,solid-liquid ratio ,extraction temperature ,and extraction times on this reflux extraction process were investigated ,to screen the influence of various factors on the yield of S. barbata total alkaloid extraction with ultraviolet spectrophotometry. Central composite design was used to design experiments ,and SAS was used to establish a prediction model ,and response surface method was used to abtain the best extraction process. Results The optimal process conditions were 40. 06 times of 55. 20%ethanol reflux extraction under 85℃for two times ,each 113. 29 min. Under the optimal process conditions , the total alkaloid content was 26. 932 9 mg/g , and the deviation of predicted values and measured values were less than 2%. The binomial fitting correlation coefficient was R2=0. 997 5. Conclusion Central-composite-design response-surface methods is successfully used for the optimization on theextraction process of S. barbatatotal alkaloids , with good predictability and stability.关键词
半枝莲/总生物碱/提取工艺/星点设计/效应面法Key words
Scutellariabarbata/total alkaloids/extraction process/central composite design/response surface method分类
医药卫生引用本文复制引用
刘欣,杨新,张丽,方振峰,张涛..星点设计-效应面法优化半枝莲总生物碱提取工艺[J].江汉大学学报（自然科学版）,2016,44(5):448-455,8.基金项目
江汉大学研究生科研创新基金 （）
