畜牧与饲料科学2016，Vol.37Issue(10)：4-7,4.
雌激素上调绵羊输卵管上皮细胞β-防御素-2基因表达中GPR30信号途径的研究
Estrogen Up-regulatesβ-defensin-2 (SBD-2) Expression in Ovine Oviduct Epithelial Cells via GPR30 Signaling Pathway
摘要
Abstract
Objective]To assess the roles of G protein-coupled receptor 30 (GPR30) in the up-regulatory effects of 17β-estrodiol (E2) onβ-defensin-2 (SBD-2) expression in ovine oviduct epithelial cells.[Methods]The 2nd passage ovine oviduct epithelial cells were treated with GPR30 agonist G1 (10-7 mol/L) and E2 (10-6 mol/L) for 6, 12, 24 h, respectively. Quantitative real-time PCR (qPCR) was used to evaluate the expression of SBD-2 gene in different observation time points. [Results] Compared with control group, the expression of SBD-2 gene in ovine oviduct epithelial cells was significantly up-regulated (P<0.01) at the 6th hour of co-treatment of G1 and E2, expressing an obvious time effect. [Conclusion]The GPR30 mediates the membrane receptor signaling pathway involved in SBD-2 gene expression up-regulation in ovine oviduct epithelial cells induced by estrogen.关键词
G蛋白偶联受体30/雌激素/输卵管上皮细胞/β-防御素-2/雌激素膜受体途径Key words
GPR30/estrogen/oviduct epithelial cells/SBD-2/estrogen membrane receptor signaling pathway分类
农业科技引用本文复制引用
包图雅,白萨日娜,杨燕燕,纳仁高娃..雌激素上调绵羊输卵管上皮细胞β-防御素-2基因表达中GPR30信号途径的研究[J].畜牧与饲料科学,2016,37(10):4-7,4.基金项目
内蒙古自然科学基金项目（2014BS0801）；内蒙古医科大学校级教学改革研究项目（ NYJXG 2016072）。 （）
