畜牧与饲料科学2016，Vol.37Issue(10)：8-9,13,3.
牛RPLP1基因的克隆和生物信息学分析
Cloning and Bioinformatics Analysis of the RPLP1 Gene in Cattle
摘要
Abstract
In the present study, the RPLP1 gene was cloned by RT-PCR assay and was subsequently bioinformatically analyzed with bioinformatics analysis software, and the tissue expression profile of this gene was determined by semi-quantitative RT-PCR method. The results showed that cattle RPLP1 gene was composed of 502 nucleotides, including a single open reading frame of 345 bp which encoded 114 amino acids; topology prediction analysis showed that there were three casein kinase Ⅱphosphorylation sites, two N-myristoylation sites and one Ribosomal-P1 conserved domain in RPLP1 protein;semi-quantitative RT-PCR assay showed that RPLP1 gene was widely expressed, and high expression was observed in skeletal, liver and cardiac muscle.关键词
克隆/RPLP1基因/牛/生物信息学/半定量RT-PCRKey words
cloning/RPLP1 gene/cattle/bioinformatics/semi-quantitative RT-PCR分类
农业科技引用本文复制引用
杨翰林,张达,张群,薛光,田万年..牛RPLP1基因的克隆和生物信息学分析[J].畜牧与饲料科学,2016,37(10):8-9,13,3.基金项目
吉林农业科技学院大学生科技创新项目（吉农院合字［2015］第052号）。 （）
