皮革科学与工程2016，Vol.26Issue(5)：21-27,7.
胶原酶活性的测定方法
On the Determination Assays for the Activity of Collagenases
摘要
Abstract
The activity of collagenases affects the depilation rate and the leather quality during enzyme unhairing due to the nature collagen could be cleaved by collagenases.The determination methods of collagenases activity were reviewed in this paper mainly including:Rosen assay,Electrophoresis assay,Suspension assay and FALGPA assay,etc.It was shown that the Rosen assay was based on ninhydrin test,which is easy operation and saves time.Chloramine-T assay was commonly used to analyze the activity of collagenase which was based on the characteristic amino acid of the collagen,hydroxyproline.However,to detect the Hyp the samples should be digested completely before the test which making the operation complex.Electrophoresis assay only could be applied to determine and compare the activity qualitatively but not quantitatively.关键词
胶原酶/酶脱毛/酶活性/底物/羟脯氨酸Key words
collagenase/enzyme unhairing/enzyme activity/substrate/hydroxyproline分类
轻工纺织引用本文复制引用
江明芳,陈敏,程海明..胶原酶活性的测定方法[J].皮革科学与工程,2016,26(5):21-27,7.基金项目
教育部重点实验室开放基金 （）
