水生生物学报2016，Vol.40Issue(6)：1095-1105,11.DOI:10.7541/2016.142
斑马鱼nrf基因时空表达分析及其在调控胰外分泌酶原基因表达中的作用研究
THE SPATIAL-TEMPORAL EXPRESSION PATTERNS OFNRF AND THEIR DIFFERENT EFFECTS ON REGULATION OF ZYMOGENS IN ZEBRAFISH
摘要
Abstract
The nuclear factor-erythroid-2 (nfe2)-related factor (Nrf) regulates genes involving in antioxidative stress re-sponse, anti-inflammatory response, and detoxification of heavy metals. Sixnrf genes (nfe2,nrf1a,nrf1b,nrf2a,nrf2b andnrf3) were found in zebrafish based on blasting of the GenBank. The phylogenetic tree analysis indicated that the vertebrate Nrf proteins were conserved during the evolution of species, in spite of the fact that thenrf1 andnrf2 respec-tively have two duplicates, resulted from the whole-genome duplication in teleosts. The results of whole mountin situ hybridization indicated that all 6 genes expressed at the one cell stage, and their expressions were ubiquitous in early stage of embryonic development. At 72 hpf,nrf1b,nrf2a,nrf2b,nrf3 andnfe2 were specifically expressed in head, part of the trunk and gut, andnrf2b was slightly expressed in the spinal cord. In general, the expressional sites of sixnrf gene were overlap. Strikingly, over-expression of Nrf1a, Nrf2a, Nrf2b and Nfe2 inducedtryl expression, however Nrf1b and Nrf3 overexpression reducedtryl expression with a dosage dependent pattern. These studies provide founda-tional data for functional superposition and complementarities among these sixnrf genes.关键词
斑马鱼/nrf/原位杂交/胰外分泌酶原基因Key words
Zebrafish/nrf/Whole-mountin situ hybridization of zebrafish/Zymogens分类
生物科学引用本文复制引用
孙素杰,谢琳琳,于翠霞,王雨薇,钟雪萍,赵浩斌,陈新华,周青春..斑马鱼nrf基因时空表达分析及其在调控胰外分泌酶原基因表达中的作用研究[J].水生生物学报,2016,40(6):1095-1105,11.基金项目
国家自然科学基金项目(31071997,31272645) （31071997,31272645）
国家海洋局海洋生物遗传资源重点实验室开放课题(HY201606)资助[Supported by the Grants from National Natural Science Foundation of China (General Program No.31071997,31272645) （HY201606）
Open Project of Key Laboratory of Marine Biological Genetic Resources of the State Oceanic Administration (HY201606) （HY201606）
