水生生物学报2016，Vol.40Issue(6)：1106-1113,8.DOI:10.7541/2016.143
GH重组蛋白对达氏鲟生长、血液生化和体组成的影响
EFFECTS OF RECOMBINANT GH ON GROWTH, BLOOD BIOCHEMISTRY, AND BODY COMPOSITION OF JUVENILE DABRY’S STURGEON ACIPENSER DABRYANUS
摘要
Abstract
In this study, Dabry’s sturgeon (Acipenser dabryanus) mature growth hormone (GH) cDNA sequence was constructed with prokaryotic expression vector pET-32a and transfected intoE.coli BL21 (DE3). RecombinantA. dabryanus GH protein (rAdGH) was expressed inE. coli cells as an inclusion body. The purified rAdGH were used as a feed additive to determine the effects of rAdGH on growth, blood biochemistry, and body composition of Dabry’s stur-geon for an eight-weeks experiment with four groups including control group (0), low-treated group (10 mg/kg), middle-treated group (50 mg/kg), and high-treated group (100 mg/kg). Results showed that the complementary rAdGH improved weight gain rate, feed efficiency, hepatosmatic index, viscera index and growth performance in the low-treated group. In addition, the low level of rAdGH treatment promoted almost all blood indexes except blood glucose. Suitable level of exogenous GH improved the content of protein and moisture in the liver and muscle, and reduced the fat content in the liver and muscle. In general, its effects on growth and metabolism were dosage-dependent. Low level rAdGH treatment significantly improved the metabolism and growth of fish, and promoted deposition of liver protein and fat consumption. However, a higher dose induced metabolic disease, inhibited growth performance, trigged liver damage and changed components. These results suggest that the recombinant GH would benefit the application of re-combinant GH in sturgeon culture.关键词
达氏鲟/原核表达/生长激素重组蛋白/生长性能/血液生化指标/体组分Key words
Acipenser dabryanus/Prokaryotic expression/Recombinant GH protein/Growth performance/Index of blood biochemistry/Body composition分类
生物科学引用本文复制引用
李创举,单喜双,岳华梅,阮瑞,危起伟..GH重组蛋白对达氏鲟生长、血液生化和体组成的影响[J].水生生物学报,2016,40(6):1106-1113,8.基金项目
公益性行业(农业)科研专项(No.201203086) （农业）
国家重点基础研究发展计划(973计划, No.2015CB150702)资助[Supported by the Fund for Agro-Scientific Research in the Public Interest (No.201203086) （973计划, No.2015CB150702）
National Basic Research Program of China (973 Program, No.2015CB150702) （973 Program, No.2015CB150702）
