水生生物学报2016，Vol.40Issue(6)：1114-1120,7.DOI:10.7541/2016.144
鄱阳湖泥鳅黑色素聚集激素1基因及其蛋白结构特征分析
STRUCTURE CHARACTERISTICS OF MELANIN-CONCENTRATING HORMONE 1 GENE AND ITS PROTEIN FROMMISGURNUS ANGUILLICAUDATUS
摘要
Abstract
Melanin-concentrating hormone (MCH) played an important role in the process of skin melanin pigmenta-tion. In order to known its characteristics and founction, the cDNA sequence of the MCH (refers toMaPmch1) from loach of the Poyang Lake was first cloned by RACE-PCR. Bioinformatics approaches were used to analyze physico-chemical property, conserved domains, secondary structures, homologous analysis and phylogenetic tree. The full length ofMaPmch1 gene was 570 bp that contained an open reading frame of 375 bp encoding 124 amino acid residues. A CpG island with the length of 110 bp in the cDNA sequence ofMaPmch1was located in 263—372 bp. It was predicted that the MaPMCH1 contained seven potential phosphorylation sites. A 17-AA-amidated cyclic neuropeptide named MaMCH1 was generated from MaPMCH1 after hydrolyzing. MaPMCH1 and MaMCH1 were pre-dicted to be hydrophilic proteins mainly contained random coil for their secondary structures. The super secondary structure of MaMCH1 with anantiparallel β-plated sheet, was connected by a disulfide bond. Homology analysis indi-cated that the amino acid sequence of MaMCH1 was highly conserved with other bony fishes. The phylogenetic tree showed that MaPMCH1 and Cyprinid fish’s PMCH1 were in the same branch, and the genetic relationship of PMCH1 was conserved with the traditional classification status. In vertebrate, RCM*GRVYRPCW (* as random amino acid) was a highly conserved sequence, suggesting thatPmch1 gene was highly conserved during the evolution.关键词
泥鳅/鄱阳湖/Pmch基因/黑色素聚集激素Key words
Misgurnus anguillicaudatus/Poyang Lake/Pmch gene/Melanin-concentrating hormone分类
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徐敏圣,史建伍,胡芳琴,肖芳美,方磊,盛军庆,王军花,洪一江..鄱阳湖泥鳅黑色素聚集激素1基因及其蛋白结构特征分析[J].水生生物学报,2016,40(6):1114-1120,7.基金项目
江西省科技支撑计划重大项目(20152ACF60013) （20152ACF60013）
江西省特种水产产业技术体系(JXARS-10) （JXARS-10）
江西省科技支撑计划(20123BBF 61121,20123BBE50096,20132BBF60052)资助[Supported by the Major Project of Jiangxi Province Science and Technology Support Program (20152ACF60013) （20123BBF 61121,20123BBE50096,20132BBF60052）
Jiangxi Province Technology System for Special Aquaculture Industries (JXARS-10) （JXARS-10）
Jiangxi Province Science and Technology Support Program (20123BBF61121,20123BBE50096,20132BBF60052) （20123BBF61121,20123BBE50096,20132BBF60052）
