水生生物学报2016，Vol.40Issue(6)：1121-1127,7.DOI:10.7541/2016.145
黄河鲤性别决定时间和相关基因表达研究
TIMING OF SEX DETERMINATION AND EXPRESSION ANALYSIS OF GENES IN YELLOW RIVER CARP (CYPRINUS CARPIO VAR.)
摘要
Abstract
The mechanisms of fish sex determination and differentiation are complex. The current study investigated the morphology and the expression of genes related to sex determination differentiation during development of Yellow River carp. Tissue sections of gonad of Yellow River carp indicated that the time of gonad derived from undifferen-tiated to stageⅡ of testis or ovary were from 40 to 80 dpf (days post-fertilization). The results showed that sex deter-mination related genes (amh,ar,cyp19a,cyp19b,dax1,dmrt1,er,foxl2,nobox,sox9a,sox9b andzp2) highly ex-pressed at 50d, especially at 45—50 dpf. The expression ofar,amh,dax1,dmrt1,sox9a,sox9b increased at 80 dpf in males, which were significantly higher than those in females, suggesting that these genes might mediate male sexual differentiation. The expression ofcyp19a,cyp19b,foxl2,nobox,zp2 increased at 80d in females, which were higher than those in males, suggesting that they might regulate female sex differentiation.关键词
性别决定/性别分化/黄河鲤Key words
Sex determination/Sex differentiation/Yellow River carp分类
生物科学引用本文复制引用
贾永芳,张弯弯,张瑞华,梁婷婷,杜启艳,常重杰..黄河鲤性别决定时间和相关基因表达研究[J].水生生物学报,2016,40(6):1121-1127,7.基金项目
国家自然科学基金项目(编号31200923和U1204329) （编号31200923和U1204329）
河南省科技厅基础与前沿技术项目(编号142300410164) （编号142300410164）
河南省科技厅科技攻关项目(编号142102310045) （编号142102310045）
河南省教育厅自然科学研究项目(编号14A180031)资助[Supported by the National Natural Science Foundation of China (No.31200923 and U1204329) （编号14A180031）
Henan Scientific and Technological Research Projects (No.142300410164) （No.142300410164）
Key Scientific and Technological Project of Henan Province (No.142102310045) （No.142102310045）
Natural Science Project of the Education Department of Henan Province (No.14A180031) （No.14A180031）
