水生生物学报2016，Vol.40Issue(6)：1144-1151,8.DOI:10.7541/2016.148
泥蚶谷胱甘肽过氧化物酶基因的全长克隆与表达分析
FULL-LENGTH CDNA CLONING AND EXPRESSION ANALYSIS OF GLUTATHIONE PEROXIDASE FROM BLOOD CLAM TEGILLARCA GRANOSA
摘要
Abstract
Glutathione peroxidase (GPx) is an important member of cellular enzymatic antioxidant system regulating stress response of host as an acute protein. In this study, a selenium-dependent glutathione peroxidase (TgGPx) gene from blood clamTegillarca granosa was cloned and analyzed by rapid amplification of cDNA ends (RACE). The nuc-leotide sequence ofTgGPx was consisted of 1195 bp with a 45 bp 5′UTR a of 511 bp 3′UTR and 639 bp open reading frame (ORF) encoding a peptide of 212 amino acids with an estimated molecular mass of 24.3 kD and a theoretical iso-electric point of 8.33.TgGPx had a characteristic codon at202UGA204 that corresponded to Selenocysteine as U53. A sel-enocysteine insertion sequence (SECIS) element was identified in the 3′-UTR ofTgGPx cDNA, which forms a stem-loop secondary structure. SECIS element plays a decisive role in the translation of the stop codon UGA into a seleno-cysteine. Multiple sequence alignment and phylogenetic analysis revealed that there were different types of GPx genes in mollusks.TgGPx has a closer phylogenic relationship with GPx1 and GPx2.TgGPx expressed in all five tissues with the highest mRNA level in mantle and the lowest in haemocytes. The expression pattern ofTgGPx in adult may sup-port its role in adult tissue growth and larval development.TgGPx was significantly up-regulated in hepatopancreas by heavy metals (Zn2+, Cu2+ and Cd2+) exposure, suggestingTgGPx may play a role in stress response and maintaining the body’s normal function inT. granosa.关键词
泥蚶/谷胱甘肽过氧化物酶/全长克隆/基因表达Key words
Tegillarca granosa/Glutathione peroxidase/Full length clone/mRNA expression分类
生物科学引用本文复制引用
程雪艳,蒋国萍,柴雪良,滕爽爽..泥蚶谷胱甘肽过氧化物酶基因的全长克隆与表达分析[J].水生生物学报,2016,40(6):1144-1151,8.基金项目
国家高技术研究发展计划(2012AA10A410-1) （2012AA10A410-1）
浙江省重大科技专项(2012C12907-4) （2012C12907-4）
温州市种子种苗科技创新专项(N20120017)资助[Supported by National High Technology Research and Development Program of China (863 Program)(2012AA10A410-1) （N20120017）
Major Science and Technology Projects of Zhejiang Province (2012C12907-4) （2012C12907-4）
Seed Science and Technology Innovation Project of Wenzhou City (N20120017) （N20120017）
