水生生物学报2016，Vol.40Issue(6)：1152-1157,6.DOI:10.7541/2016.149
热应激对齐口裂腹鱼非特异性免疫功能及细胞凋亡的影响
EFFECTS OF HEAT STRESS ON SOME NON- SPECIFIC IMMUNE FACTORS AND BLOOD CELL APOPTOSIS INSCHIZOTHORAX PRENATI
摘要
Abstract
Schizothorax prenati, an important cold-water fish in China, is a precious commercial fish in the upper Yangtze River. The current study investigated the effects of heat stress and recovery on the damage of organism, the functions of non-specific immunity and apoptosis inSchizothorax prenati under 3 conditions: before heat stress (19°C, control group), after heat stress (27°C for 0, 2, 4, 8 and 12h, respectively; experimental groups) and 6h after heat stress recovery (19°C recovery group). The results indicated that the activity of superoxide dismutase (SOD) increased at 0, 4, 8 and 12h after stress, and it increased significantly at 0, 12h compared with the pre-stress (P<0.01). The activity of my-eloperoxidase (MPO) reached the maximum level at 4h. The activity of glutamic-oxalacetic transaminase (GOT) in all experimental groups had no significant difference compared with the control group, and it was significantly higher than that in the control group at 6h after recovery (P<0.01). The activity of glutamic-pyruvic transaminase (GPT) at 0, 4, 12h after stress and 6h after recovery was significantly higher than that in the control group (P<0.01). The content of malonaldehyde (MDA) at 2, 4, and 12h after heat stress was significantly higher than that in the control group (P<0.01), and it was significantly higher than that in the pre-stress at 8h (P<0.05), which reached the maximum level at 12h after stress, and the content of MDA at 6h after recovery returned to pre-stress levels (P<0.05). C3 complement (C3) level kept rising and was significantly higher than that of the control group at 12h under heat stress (P<0.05), but it de-creased at 6h after recovery having significant difference from the control group (P>0.05). Compared with the pre-stress group, apoptosis rate in all experimental groups was significantly higher after heat stress (27) (P<0.05) and reached the maximum level (52.42%) at 4h after treatment, while declined markedly at 6h after recovery. These results indicated that heat stress changed the non-specific immunity of the organism and induced inflammation and induced cells damage, but all functions can recover gradually after the temperature returning to the moderate level. The results provided basic data for further research on the mechanism of high temperature adaption inSchizothorax prenati, as well as a scientific basis for temperature control during cultivation and transit of this species.关键词
齐口裂腹鱼/热应激/非特异性免疫/细胞凋亡Key words
Schizothorax prenati/Heat stress/Non-specific immunity/Apoptosis分类
农业科技引用本文复制引用
黄正澜懿,陈世静,张争世,向梦斌,罗辉,叶华..热应激对齐口裂腹鱼非特异性免疫功能及细胞凋亡的影响[J].水生生物学报,2016,40(6):1152-1157,6.基金项目
国家自然科学基金项目(31402302) （31402302）
中央高校基本科研业务费项目(XDJK2014C152) （XDJK2014C152）
西南大学荣昌校区青年基金项目(20700919)资助[Supported by the National Natural Science Foundation of China (31402302) （20700919）
Fundamental Research Funds for the Central Universities (XDJK2014C152) （XDJK2014C152）
the Youth Foundation of Southwest University Rongchang Campus (20700919) （20700919）
