水生生物学报2016，Vol.40Issue(6)：1166-1171,6.DOI:10.7541/2016.151
一种新型草鱼呼肠孤病毒人工感染方法
PRELIMINARY STUDY ON A NEW METHOD OF GCRV ARTIFICIAL INFECTION
摘要
Abstract
Grass carp (Ctenopharyngodon idella) is an important freshwater economic fish in China and its production was 5.5×109 kg in 2014, accounting for 13% of global freshwater fishery production, which was the highest output and consumption of freshwater fish worldwide. Hemorrhagic disease caused by grass carp reovirus (GCRV) seriously af-fects the grass carp production. This study isolated and purified a new strain of GCRV from diseased grass carp from muscle of dead fish with typical symptoms of hemorrhagic diseases, whihc was further determined.by electron micro-scope and RT-PCR. A large number of virus particles with 70—80 nm in diameter were observed by electron micro-scope, which was diagnosed as genotype (GCRV-) by RT-PCR. The titer of virus was determined by absolute quantit-ative PCR (2.97×103 copy/μL). The GCRV artificial infection experiments with 3 three different concentrations and one PBS control group were conducted with new method of lavage. The mortality rate of three experimental groups were all about 80%, while only one fish died in PBS control group. The typical symptoms of hemorrhagic disease were found in the abdomen, fin base and gill cover of dead fish. GCRV- was detected from four randomly selected dead fish using RT-PCR. These results indicate that lavage could be a new effective way for GCRV artificial infection expe-riment.关键词
草鱼呼肠孤病毒/人工感染/水产养殖/RT-PCR/分型Key words
Grass carp reovirus/Artificial infection/Aquaculture/RT-PCR/Classification分类
生物科学引用本文复制引用
褚鹏飞,何利波,汪浩,陈庚,李勇明,黄容,廖兰杰,朱作言,汪亚平..一种新型草鱼呼肠孤病毒人工感染方法[J].水生生物学报,2016,40(6):1166-1171,6.基金项目
国家自然科学基金重点项目(No.31130055) （No.31130055）
淡水生态与生物技术国家重点实验室自主课题(No.2014FBZ040)资助[Supported by the National Natural Science Foundation of China (No.31130055) （No.2014FBZ040）
State Key Laboratory of Freshwater Ecology and Biotechnology (No.2014FBZ04) （No.2014FBZ04）
