天然产物研究与开发2016，Vol.28Issue(9)：1351-1356,6.DOI:10.16333/j.1001-6880.2016.9.002
羊肚菌多糖的分离纯化及其体外抗氧化、抗肿瘤活性研究
Isolation, Purification, Antioxidant and Antitumor Activities of Polysaccharides from Morchella esculenta
摘要
Abstract
Two water-soluble polysaccharides (MEP1-1 and MEP2-3) were extracted and purified using chromatography on DEAE-52 and Sephadex G-200 column from Morchella esculenta.Their chemical composition and in vitro antioxidant and antitumor activities were investigated.The results suggested that the molecular weights of MEP 1-1 and MEP 2-3 were approximately 1.37 × 105 Da and 1.15 × 104 Da,respectively.Characteristic bands in FT-IR spectra indicated both MEP1-1 and MEP2-3 had a small amount ofβ-glycosidic bond.Antioxidant activities of MEP1-1 and MEP2-3 were both higher than crude MEP.MEP2-3 had higher antioxidant activities on hydroxyl radicals than MEP1-1 while MEP1-1 had higher antioxidant activities on superoxide radicals than MEP2-3.MEP 1-1 fraction appeared higher anticancer activity against HeLa cells than MEP2-3 fraction,with IC50 values of 29 μg/mL.MEP2-3 fraction showed higher anticancer activity against A549 cells than MEP 1-1 fraction,with IC50 values of 81 μg/mL at 72 h.It could be explored as a potential adjuvant against cancer used in the health food and pharmaceutical therapy.关键词
羊肚菌/多糖/抗氧化活性/抗肿瘤活性Key words
Morchella esculenta/polysaccharides/antioxidant activity/antitumor activity分类
农业科技引用本文复制引用
李敬,周伟杰,赵士豪,吕慧丽..羊肚菌多糖的分离纯化及其体外抗氧化、抗肿瘤活性研究[J].天然产物研究与开发,2016,28(9):1351-1356,6.基金项目
河北省自然科学基金(B2016207013) （B2016207013）
河北省高等学校科学研究计划(QN2016268) （QN2016268）
河北经贸大学校内基金重点项目(2014KYZ01,2016KYZ02) （2014KYZ01,2016KYZ02）
河北省科技支撑计划(14236802D-2) （14236802D-2）
河北经贸大学教学研究项目(2016JYQ06,2016JYZ03) （2016JYQ06,2016JYZ03）
