天然产物研究与开发2016，Vol.28Issue(9)：1357-1362,6.DOI:10.16333/j.1001-6880.2016.9.003
可作为疫苗佐剂的云芝多糖磷酸钙纳米粒的制备与表征
Preparation and Characterization of Polysaccharide Krestin Calcium Phosphate (PSK-CAP) Nanoparticle as a Vaccine Adjuvant
摘要
Abstract
We hypothesize that calcium phosphate (CAP) coated polysaccharide as vaccine adjuvant can improve the transmission efficiency as well as enhance immune response.In this study,unpurified polysaccaride krestins (PSK) was treated in traditional craft.PSK was then oxidized by sodium periodate to obtain a multi-aldehyde product.PSK-aldehyde was reacted with pamidronate (PA) to obtain Pa-modified PSK (PSK-PA) and the products were identified by infrared spectrum (IR).PSK-PA was used to synthesize CAP nanoparticles by co-precipitation method.The size and PDI was used to optimize the formulation of the nanoparticle.The morphology of nanoparticles was observed using scanning electron microscope (SEM).Subsequently,ovalbumin (OVA),used as a model antigen,was loaded into the nanoparticles to examine the cytotoxicity on RAW264.7.The uptake of PSK-CAP-OVA-FITC loaded nanoparticle was investigated on RAW264.7 by fluorescent microscopy.The average diameter of the nanoparticles was about 100 nm with a PDI less than 0.3 indicating a homogeneous dispersion.Interestingly,the toxicity of PSK-CAP-OVA nanoparticles was significantly re-duced when compared to the free PA.Additionally,we observed that Raw264.7 cell preferentially uptake the PSK-CAP-OVA nanoparticles compared to that of the free OVA.In conclusion,PSK-CAP nanoparticles can provide a reference for future studies on immune adjuvants.关键词
云芝多糖/磷酸钙纳米粒/免疫佐剂/OVA/纳米粒表征Key words
PSK/calcium phosphate nanoparticles/vaccine adjuvant/OVA/nanoparticle characterization分类
医药卫生引用本文复制引用
宋志磊,潘玉涛,黄新河,刘新荣,晏为力..可作为疫苗佐剂的云芝多糖磷酸钙纳米粒的制备与表征[J].天然产物研究与开发,2016,28(9):1357-1362,6.基金项目
四川省科技厅项目(2015JY0202) （2015JY0202）
成都市科技惠民项目(2015-HM01-00047-SF) （2015-HM01-00047-SF）
