天然产物研究与开发2016，Vol.28Issue(9)：1367-1371,5.DOI:10.16333/j.1001-6880.2016.9.005
Desmosdumotin-C A环衍生物TEP诱导HL-60细胞凋亡作用机制研究
The Effects and Mechanisms of an A ring Derivative of Desmosdumotin-C (TEP) induced Apoptosis in HL-60 Cells
摘要
Abstract
This study focused on the effects of TEP,an A ring derivative of desmosdumotin-C,on the human acute leukemia HL-60 cell apoptosis,and the underlying mechanisms.TEP-induced apoptosis and its effect on Fas,FasL,Bax,Bcl-2 expression were measured by flow cytometry,and the morphological changes of the apoptosis cells were further observed by transmission electron microscop.The results showed that 24 h after 40 μg/mL TEP treatment,the typical apoptotic morphological changes were induced in HL-60 cell.Meantime,40 μg/mL TEP significantly increased Fas,FasL,and Bax expression (P ＜ 0.05),and decreased Bcl-2 expression (P ＜ 0.05).All the results suggested that TEP effectively induced apoptosis in HL-60 cells,and the mechanism may be involved in upregulating Fas,FasL,Bax expression,and reducing Bcl-2 expression.关键词
毛叶假鹰爪素C/衍生物/HL-60/凋亡/作用机制Key words
desmosdumotin C/derivative/HL-60 cell/apoptosis/mechanism分类
医药卫生引用本文复制引用
郭宏举,向卓,常李荣,史宁,梁海,郑凯音,吴久鸿..Desmosdumotin-C A环衍生物TEP诱导HL-60细胞凋亡作用机制研究[J].天然产物研究与开发,2016,28(9):1367-1371,5.基金项目
国家自然科学基金(81072546 （）
81470156) （）
