天然产物研究与开发2016，Vol.28Issue(9)：1377-1383,7.DOI:10.16333/j.1001-6880.2016.9.007
黄芩苷对LPS诱导的家兔乳腺炎的保护作用
Protective Effect of Baicalin on LPS Induced Inflammatory Responses in Rabbit Mammary Gland Tissue
摘要
Abstract
The aim of this study was to investigate protective effect of baicalin on LPS induced rabbit mastitis.Fifty healthy rabbits after parturition 7 ～ 10 d were divided into five groups randomly,including mastitis model control group (LPS,0.25 mg/kg · BW),baicalin groups with the dose 75,150 and 300 mg/kg· BW respectively,and dexamethasone (DEX) group with dose of 0.5 mg/kg · BW DEX.LPS was respectively infused into the fourth mammary glands of rabbit via the teat duct.In the baicalin and DEX groups,baicalin and DEX were injected through the auricular veins at 48 h,24 h,1 h before LPS infusion and after 8 h,32 h,56 h respectively.The rectal temperature was measured and the auricular veins blood were collected 50 h before,and 6,12,24,48,72 h after LPS infusion respectively.The following indexes at different time point were determined:(1) The pathological change of rabbit mammary gland tissues.(2) The activities of ALP,LDH,myeloperoxidase (MPO) and N-Acetylβ-D-Glucosaminidase (NAG) in serum.(3) The levels of TNF-α,interleukin-1 (IL-1) and interleukin-6 (IL-6) in serum were measured by ELISA.The results showed that:(1) Baicalin could reduce the histopathological damage.(2) Baicalin could reduce the activities of ALP,LDH,MPO and NAG.Baicalin group (300 mg/kg · BW) had the best effect of decreasing in the activities of ALP,LDH,MPO and NAG.(3) Baicalin could reduce the level of TNF-α,IL-1,and IL-6 in serum.The level of TNF-α in serum was significantly lower than model control group in 6 h (P ＜ 0.05).The level of IL-1 in serum was significantly lower than model control group in 72 h (P ＜ 0.05).The results demonstrated that baicalin can reduce the histopathological damage and relief body symptom.The highest potent protective effect of baicalin on LPS induced rabbit mastitis was showed at the dose of 300 mg/kg · BW.关键词
乳腺炎动物模型/黄芩苷/家兔/脂多糖/抗炎作用Key words
mastitis model of animal/baicalin/rabbit/LPS/anti-inflammatory activity分类
农业科技引用本文复制引用
文月玲,张于,姚学萍,沈留红,余树民,曹随忠..黄芩苷对LPS诱导的家兔乳腺炎的保护作用[J].天然产物研究与开发,2016,28(9):1377-1383,7.基金项目
四川省科技支撑计划(2013NZ0032) （2013NZ0032）
四川农业大学学科建设双支计划(3570806) （3570806）
