天然产物研究与开发2016，Vol.28Issue(9)：1384-1389,1383,7.DOI:10.16333/j.1001-6880.2016.9.008
苦马豆素诱导人喉癌Hep-2细胞凋亡研究
Effect of Swainsonine on Apoptosis of Human Laryngeal Cell Line Hep-2
摘要
Abstract
Swainsonine have a wide range of antitumor and immunomodulatory effects.The apoptosis-inducing effect of swainsonine on human laryngeal carcinoma Hep-2 cells and its mechanisms were investigated in this study.Different concentrations of swainsonine was applied to Hep-2 cells for 24 h,the cells activity was detected by MTT assay.Cells morphology was observed under a fluorescence microscope.Cell apoptosis was detected by Annexin V-FITC/PI double labeling.JC-1 was used to detect the mitochondrial membrane potential.The mRNA and protein levels of Bax and XIAP were measured by Real-time PCR and Western blot respectively.Swainsonine inhibited the proliferation and induced the apoptosis of Hep-2 cells in a dose-dependent manner in a certain concentration range.The different levels of morphology change of apoptosis were observed.The eversion of phosphatidyl serine was aggravatingly intensified,and mitochondrial membrane potential decreased.The mRNA and protein expression level of Bax increased.The mRNA and protein expression level of XIAP decreased.Hence,it was concluded that swainsonine induced apoptosis through the mitochondrial pathway and dependent on the regulation of the proteins levels of Bax and XIAP.关键词
苦马豆素/Hep-2细胞/细胞凋亡/线粒体途径Key words
swainsonine/Hep-2 cells/apoptosis/mitochondrial pathway分类
生物科学引用本文复制引用
李莎,张思楠,杨军,伍春莲..苦马豆素诱导人喉癌Hep-2细胞凋亡研究[J].天然产物研究与开发,2016,28(9):1384-1389,1383,7.基金项目
中国博士后基金(2013M540391) （2013M540391）
四川省教育厅重大培育项目(13CZ0029) （13CZ0029）
三峡库区生态环境与生物资源省部共建重点实验室开放课题(SKL-2011-05) （SKL-2011-05）
