天然产物研究与开发2016，Vol.28Issue(9)：1390-1396,1413,8.DOI:10.16333/j.1001-6880.2016.9.009
SPME-GC/MS联合HSDE-HPLC-DAD分析表征金银花新品种中的主要化学成分
Analysis and Characterization of the Chemical Ingredients of Lonicera japonica by SPME-GC/MS Coupled with HSDE-HPLC-DAD
摘要
Abstract
In this study,a comprehensive method was established to analyze and characterize the chemical ingredients in a new variety of Lonicera japonica by SPME-GC/MS coupled with HPLC-DAD.The volatile oils of the investigated sample were determined by GC/MS.Other organic acids,flavonoids and iridoids were analyzed by HSDE-HPLC-DAD.As a result,54 volatile oils were detected.In addition,the contents of four organic acids (chlorogenic acid,cryptochlorogenic acid,isochlorogenic acid A and isochlorogenic acid C),two flavonoids (rutin and galuteolin) and two iridoids (loganic acid and chiratin) were determined.This study proposed a method for the comprehensively and systematically evaluation of germplasm by determining the 4 types of main ingredients in L.japonica.It will provide scientific basis for the popularization and further utility of L.japonica.关键词
GC/MS/HPLC-DAD/金银花/挥发油类/黄酮类/有机酸类/环烯醚萜苷类Key words
GC/MS/HPLC-DAD/Lonicera japonica/volatile oil/organic acid/flavonoid/iridoid分类
医药卫生引用本文复制引用
张敏敏,刘代成,王岱杰,耿岩玲,王晓,赵恒强..SPME-GC/MS联合HSDE-HPLC-DAD分析表征金银花新品种中的主要化学成分[J].天然产物研究与开发,2016,28(9):1390-1396,1413,8.基金项目
国家自然科学基金(81473298) （81473298）
山东省攻关计划(2014GSF119031) （2014GSF119031）
山东省自然科学基金(ZR2013HL029) （ZR2013HL029）
山东省科学院青年基金(2014-5) （2014-5）
