天然产物研究与开发2016，Vol.28Issue(9)：1397-1401,1371,6.DOI:10.16333/j.1001-6880.2016.9.010
超高效液相色谱-三重四级杆质谱同时测定灯盏细辛中10种指标成分的含量
Simultaneous Determination of Ten Index Compounds in Erigeron breviscapus Using UPLC-MRM-MS
摘要
Abstract
An ultra-high performance liquid chromatography-multiple reactions monitoring-mass spectrometry (UPLC-MRM-MS) method was established for the simultaneous determination of ten components namely chlorogenic acid,neochlorogenic acid,cryptochlorogenic acid,scutellarin,apigenin-7-O-glucronide,apigenin,caffeic acid,3,4-dicaffeoylquinic acid,3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid in Erigeron breviscapus.A waters BEH C18 column (2.1 mm × 50 mm,1.7 μm) was used for the chromatographic separation with a gradient solvent system of 0.1％ formic acid in acetonitrile (A)-0.1％ formic acid in water (B) as mobile phases.MRM mode was used as detection mode with electro-spray ionization (ESI) source.In certain range of concentrations,good linear relationships were established for the ten analytes.The average recoveries were 97.65％ ～ 105.4％,RSD were 0.66％ ～ 5.9％.The precision,repeatability and stability were good.The results showed that the contents of scutellarin components in E.breviscapus were all beyond the standard in Pharmacopoeia of the People's Republic of China.The developed UPLC-MRM-MS method was simple,rapid and effective for the determination of the components in E.breviscapus.It provided a reliable way to control the quality of E.breviscapus.关键词
超高效液相色谱-三重四级杆质谱/灯盏细辛/指标成分/含量测定Key words
UPLC-MRM-MS/Erigeron breviscapus/index compounds/content determination分类
医药卫生引用本文复制引用
侯佳,邹欢,李勇军,巩仔鹏,王爱民..超高效液相色谱-三重四级杆质谱同时测定灯盏细辛中10种指标成分的含量[J].天然产物研究与开发,2016,28(9):1397-1401,1371,6.基金项目
国家自然科学基金(81260636) （81260636）
贵州省科学技术基金(黔科合J字[2013]2035号) （黔科合J字[2013]2035号）
贵州省中医药管理局项目(QZZY-2015-080) （QZZY-2015-080）
贵州省学位委员会项目(黔教研合[2014-1871]) （黔教研合[2014-1871]）
贵州省优秀青年科技人才培养对象专项资金(黔科合人字[2015]11号) （黔科合人字[2015]11号）
贵州省教育厅项目(黔教合协同创新字[2013]04) （黔教合协同创新字[2013]04）
