烟草科技2016，Vol.49Issue(10)：9-14,6.DOI:10.16135/j.issn1002-0861.2015.0642
宜宾烟区烤烟野火病的致病力分化、生物学特性及抑菌药剂筛选
Pathogenicity differentiation and biological characteristics of Pseudomonas syringae pv. tabaci and effective bactericide screening in Yibin tobacco planting areas
摘要
Abstract
To control Pseudomonas syringae pv. tabaci more effectively, pathogen was isolated from the tobacco leaves with typical wildfire symptom in Yibin tobacco planting areas. The isolated pathogen was cultured to observe its morphological characteristics. The pathogenicity of pathogen strains was evaluated via acupuncture inoculation and injection inoculation without needle. The biological characteristics of representative strains [pv-xs-y-2 (strong), pv-xt-y-2 (moderate) and pv-gy-y-1 (weak)] with different pathogenicity were determined by measuring bacteria growth rate, and the inhibition effects of four bactericides on strong pathogenicity strain pv-xs-y-2 were investigated. The results showed that the morphological characteristics of fourteen strains were consistent with those of P. syringae pv. tabaci, but the pathogenicity was significantly different between strains with the average disease index in the range of 23.33-72.22. Based on the pathogenicity of K326, the fourteen strains were clustered into three groups: strong (disease index 60-72.22), moderate (disease index 34.44-53.33) and weak (disease index 23.33); in which, the strains with moderate pathogenicity accounted for 71.43%. On NA medium, the optimum growth temperature for pathogen was 28 ℃, at which the OD600 values of pv-xs-y-2, pv-xt-y-2 and pv-gy-y-1 were 1.285, 1.105 and 0.977, respectively; the optimum growth relative humidity was 100%, at which their OD600 values were 0.694, 0.555 and 0.505, respectively; the optimum growth pH was 7. The data indicated that neutral environment was suitable for the growth of P. syringae pv. tabaci. The inhibition effects of different bactericides on P. syringae pv. tabaci were 3% benziothiazolinone>4% kasugamycin>72% streptomycin>20% thiediazole copper, and EC50 value of 3% benziothiazolinone was 9.401 µg·mL-1.关键词
烤烟/野火病/致病性/生物学特性/病情指数/杀菌剂/抑制效果Key words
Flue-cured tobacco/Pseudomonas syringae pv. tabaci/Pathogenicity/Biological characteristic/Disease index/Bactericide/Inhibition effect分类
农业科技引用本文复制引用
唐明,杨洋,易蔓,谢冰,程智敏,向金友,饶在生,蔡毅,黄胜,杨苹,杨懿德..宜宾烟区烤烟野火病的致病力分化、生物学特性及抑菌药剂筛选[J].烟草科技,2016,49(10):9-14,6.基金项目
四川省烟草公司资助项目“烤烟赤星病和野火病综合防治技术研究与示范”（川烟科［2014］6号）。 （）
