应用生态学报2016，Vol.27Issue(10)：3371-3378,8.DOI:10.13287/j.1001-9332.201610.017
一株敌草胺降解菌的分离鉴定及降解性能
Identification and degradation characteristics of a napropamide-degrading bacterium strain
摘要
Abstract
In order to investigate the microbial degradation mechanism of amide herbicide napropamide and its degradation bioaugmentation in soil,a bacterial strain LGY06 capable of utilizing napropamide as sole carbon and energy source was isolated from a tobacco-planted soil after successive application of napropamide.LGY06 was identified as Bacillus cereus based on morphological,physiological and biochemical characteristics,and the 16S rDNA homologue sequence analysis.The degradation of napropamide in pure cultures by LGY06 was fitted to the first-order function.The strain LGY06 could degrade more than 75.7％ of 50 mg · L-1 napropamide within 7 d.The optimal temperature and pH for napropamide degradation was 35 ℃ and 8.0,respectively.The pathway of napropamide degradation was elucidated based on metabolites identification by GC-MS.The main degradation products of napropamide by LGY06 were α-naphthol and propylacetanilide.The metabolism of napropamide by strain LGY06 involved dealkylation and oxidation (or hydrolyzation).Under the laboratory control conditions,the bacterial strain LGY06 could effectively enhance the degradation of napropamide in soil.Compared with the un-inoculated controls,the half-life of napropamide in sterilized soil,non-rhizosphere soil,and rhizosphere soil inoculated with the strain LGY06 was shorted by 79.5％,36.6％ and 41.1％,respectively.关键词
敌草胺/菌株LGY06/蜡状芽孢杆菌/降解性能/降解途径Key words
napropamide/strain LGY06/Bacillus cereus/degradation characteristics, degradation pathway引用本文复制引用
张承,吴小毛,龙友华,李明,李荣玉,尹显慧..一株敌草胺降解菌的分离鉴定及降解性能[J].应用生态学报,2016,27(10):3371-3378,8.基金项目
本文由国家自然科学基金项目(21267007)、贵州省科技厅农业攻关项目([2009]3022,[2011]3024,[2012]3010号)、中国烟草总公司贵州省公司科技项目(201311,201414,201508,201509)和贵州省科学技术基金项目([2010]2074)资助 This paper was supported by the National Natural Science Foundation of China(21267007),the Agricultural Research Project of Guizhou Province([2009]3022,[2011]3024,[2012]3010),the Science Project of Guizhou Branch of China National Tobacco Corp (20131,201414,201508,201509) and the Science and Technology Foundation of Guizhou Province([2010]2074). （21267007）
