中国当代儿科杂志2016，Vol.18Issue(11)：1174-1180,7.DOI:10.7499/j.issn.1008-8830.2016.11.023
孟鲁司特钠对哮喘小鼠气道重塑及Th17/CD4+CD25+Treg表达的影响
Effect of montelukast sodium intervention on airway remodeling and percentage of Th17 cells/CD4+CD25+regulatory T cells in asthmatic mice
摘要
Abstract
Objective To study the dynamic changes in the percentage of Th17 cells/CD4+CD25+regulatory T cells after intervention with montelukast sodium, a leukotriene receptor antagonist, in asthmatic mice and the association between them. Methods Balb/c mice were randomly divided into blank group, asthma group, and montelukast sodium group. The asthmatic mouse model of airway remodeling was established by sensitization with intraperitoneal injection of chicken ovalbumin (OVA) and aluminum hydroxide suspension and aerosol inhalation of OVA. The mice in the blank group were given normal saline, and those in the montelukast sodium group were given montelukast sodium by gavage before aerosol inhalation. Eight mice were randomly sacriifced within 24 hours after 2, 4, and 8 weeks of aerosol inhalation. The pathological sections of lung tissue were used to observe the degree of airway remodeling. Flow cytometry was used to measure the percentages of Th17 cells and CD4+CD25+regulatory T cells in CD4+T cells. Results The asthma group and the montelukast sodium group had signiifcantly higher bronchial wall thickness and smooth muscle thickness at all time points compared with the blank group (P<0.05). At 8 weeks of intervention, the montelukast sodium group had significantly greater improvements in the above changes compared with the asthma group (P<0.05). Compared with the blank group, the asthma group and the montelukast sodium group had significant increases in Th17 cells (positively correlated with airway remodeling) and significant reductions in CD4+CD25+ regulatory T cells (negatively correlated to airway remodeling) at all time points (P<0.05). At 8 weeks of intervention, the montelukast sodium group had a significant reduction in the number of Th17 cells and a significant increase in the number of CD4+CD25+ regulatory T cells compared with the asthma group (P<0.05). Conclusions Montelukast sodium intervention can alleviate airway remodeling and achieve better improvements over the time of intervention. The possible mechanism may be related to the improvement of immunologic derangement of CD4+CD25+ regulatory T cells and inhibition of airway inflammation.关键词
哮喘/白三烯受体拮抗剂/Th17细胞/CD4+CD25+Treg细胞/小鼠Key words
Asthma/Leukotriene receptor antagonist/Th17 cell/CD4+CD25+regulatory T cell/Mice引用本文复制引用
李丽,娄春艳,李敏,张剑波,陈杰..孟鲁司特钠对哮喘小鼠气道重塑及Th17/CD4+CD25+Treg表达的影响[J].中国当代儿科杂志,2016,18(11):1174-1180,7.基金项目
四川省卫生厅科研项目（070089）。 （）
