应用巢式PCR检测孕妇血浆中cffDNA RHD基因型的研究OA北大核心CSTPCD
The study of fetal RHD genotyping with cffDNA in Rh negative maternal plasma by nested PCR
目的 建立巢式PCR技术检测RhD阴性孕妇血浆中游离胎儿DNA(cffDNA)的RHD基因型,以预测胎儿RhD血型.方法 采用QIAamp DNA Blood Mini Kit提取32例RhD阴性孕妇血浆游离DNA,针对RHD外显子7和10分别设计外侧、内侧2组特异性引物,巢式PCR方法检测孕妇血浆游离胎儿DNA的RHD型,测序验证PCR产物的序列特异性.结果 孕妇血浆游离DNA经巢式PCR扩增后,有27例成功扩增出RHD外显子7、10特异性条带…查看全部>>
Objective To establish a nested PCR method to detect fetal RhD genotype in order to predict fetal RhD blood type.Methods The cell free fetal DNA was extracted from RhD negative maternal plasma by using QIAamp DNA Blood Mini Kit.Two groups of specific outside and inside primers aimed at RHD exon 7 and 10 were designed and the nested PCR was carried out to detect fetal DNA RHD genotype.The specificity of nested PCR product was verified by sequencing.Resu…查看全部>>
陈利萍;左琴琴;徐华;周华友;张印则
陕西省血液中心,陕西西安710061陕西省血液中心,陕西西安710061陕西省血液中心,陕西西安710061Rh血型系统基础与临床研究关键技术攻关合作组南方医科大学南方医院
临床医学
巢式PCR血浆游离胎儿DNARHDRhD新生儿溶血
nested PCRcell free fetal DNARHDRh hemolytic disease of newborn
《中国输血杂志》 2016 (8)
785-787,3
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