摘要
Abstract
Objective To investigate the peripheral blood T lymphocyte subsets in invalid red blood cell transfusion cases and to observe regulatory T cells (T-reg cells) level changes.Methods Flow cytometry was used before and after respectively to detect the peripheral blood T lymphocyte subsets (CD3 +,CD4+,CD8 +,CD4 +/CD8 +,CD4 + CD25 + Treg) and their levels in 30 patients with invalid red blood cell infusion.Results In invalid transfusion cases,the number of CD3 + T cell and CD4+T cells (P ＜ 0.05) and the CD4+/CD8 + ratio rose before blood transfusion.The percentage of CD8+T cells did not change (P ＞ 0.05).The CD4 + CD25 + T-reg levels fell before a blood transfusion.In the successful transfusion group,the level of T lymphocyte subsets did not change before and after blood transfusion (CD3 +,CD4 +,CD8 +,CD4 +/CD8 +,CD4 + CD25 + Treg) (P ＞ 0.05).Conclusion In invalid transfusion cases,patients may harbor abnormal CD4 + CD25 + T-reg cells,which can lead to abnormal activation of T cells and may contribute to the failure of red blood cell transfusion.关键词
红细胞输注无效/T淋巴细胞亚群/CD4+ CD25+ Treg细胞/流式细胞仪Key words
invalid red blood cell transfusion/T lymphocyte subsets/CD4+ CD25+ regulatory T cell/flow cytometry分类
临床医学
