中国实验动物学报2016，Vol.24Issue(5)：448-453,6.DOI:10.3969/j.issn.1005-4847.2016.05.002
基因沉默组织型转谷氨酰胺酶抑制SaOS-2细胞成骨分化
Knockdown of tissue transglutaminase in SaOS-2 cell line inhibits its osteoblastic differentiation and mineralization
摘要
Abstract
Objective To investigate whether TG2 plays an important role in the osteoblast differentiation and mineralization.Methods TG2 mRNA of SaOS-2 cells was knocked down using a lentivirus stably expressing short-hairpin ( sh) RNA targeting TG2.Then the cells were cultured in osteo-inductive medium for 14 d to measure mineralization and for 7 d to measure the levels of osteoblastic differentiation markers including ALP activity and mRNA of collagen I, osteocalcin ( OCN) and BMP-2.The wild-type SaOS-2 cells and scrambled shRNA-transducted SaOS-2 cells served as the controls. Results The controls displayed an increasing trend of the level of ALP activity and mRNA of collagen I, osteocalcin and BMP-2,and notable mineralization at 14 d.When TG2 was knocked down, ALP activity, mRNA of collagen I, osteocalcin and BMP-2 at 7d,and mineralization at 14 d were all significantly lower in comparison with the corresponding values in the controls.Conclusion TG2 is involved in the differentiation and mineralization of osteoblasts in vitro.关键词
组织型转谷氨酰胺酶/SaOS-2细胞/成骨分化Key words
Tissue transglutaminase/SaOS-2 cell line/Osteoblastic differerntiation分类
生物科学引用本文复制引用
余慧镭,殷晓雪,陈仲强,冷慧杰,宋纯理,刘忠军..基因沉默组织型转谷氨酰胺酶抑制SaOS-2细胞成骨分化[J].中国实验动物学报,2016,24(5):448-453,6.基金项目
国家自然科学基金（编号81101334）。 （）
