中国实验动物学报2016，Vol.24Issue(5)：454-459,6.DOI:10.3969/j.issn.1005-4847.2016.05.003
比较谷氨酸引起两品种大鼠培养星形胶质细胞肿胀的差异
Comparison of the differences in glutamate-induced astrocyte swelling between Wistar and Sprague-Dawley rats
摘要
Abstract
Objective To compare the differences between the cell swelling of cultured astrocytes ( AST) from Wistar and Sprague-Dawley ( SD) rats after incubation with glutamate.Methods Primary cultured AST derived from the cerebral cortex of one-day-old Wistar or SD rats were prepared.The cultured AST received 1 or 10 mmol/L glutamate treat-ment for 48 h on the tenth day after subculture.The viability of AST was determined by lactate dehydrogenase ( LDH) kit to assess the cell injury, and the perimeter of AST was measured using Image Pro Plus software after glial fibrillary acidic protein immunofluorescence staining to evaluate the astrocyte swelling.Then, the expression of aquaporin 4 ( AQP4 ) in cultured AST was detected by quantitative reverse transcription polymerase chain reaction.Results No significant differ-ence was found in the LDH release after the glutamate treatment in cultured AST from these two strains (P>0.05).The perimeter of AST from normal Wistar rats was shorter than that from SD rats, but was longer after the treatment of glutamate (P<0.05).Meanwhile, AQP4 expression in the Wistar rats was significantly higher than that from SD rats after incuba-tion with 1 mmol/L glutamate ( P<0.05 ) .Conclusions These results suggeste that cultured AST from Wistar rats are more susceptible to glutamate-induced swelling than that from SD rats, and there are differences between the effects of glu-tamate on AQP4 expression in astrocytes of Wistar and SD rats.关键词
谷氨酸/星形胶质细胞/水通道蛋白4/大鼠Key words
Glutamate/Astrocyte/Aquaporin 4/Rats分类
生物科学引用本文复制引用
师忠芳,徐立新,卢易,董丽萍,闫旭,杨少华,袁芳..比较谷氨酸引起两品种大鼠培养星形胶质细胞肿胀的差异[J].中国实验动物学报,2016,24(5):454-459,6.基金项目
国家自然科学基金（NO．81271286，81228009）；北京市自然科学基金面上项目（项目资助号7152027）。 （）
