中国比较医学杂志2016，Vol.26Issue(10)：19-24,6.DOI:10.3969.j.issn.1671-7856.2016.10.005
慢性心力衰竭大鼠肝脏组织的代谢轮廓
Metabolic profiling of liver in the rats with chronic heart failure
摘要
Abstract
Objective To establish a rat model of liver metabolism profile in chronic heart failure (CHF), to explore the dynamics of liver metabolism in CHF from the point of view of metabolism, and to find the characteristic metabolites valuable for the molecular mechanism and management of CHF.Methods Twenty male Wistar rats were assigned to the CHF group to receive aortic coarctation or to the control group to receive sham surgery, and were bred for 24 weeks following surgery.The metabolic profiling of the rat liver tissues was analyzed on a metabonomics research platform. Orthogonal partial least squares-discriminant analysis ( OPLS-DA) model and principal component analysis ( PCA) model were established for liver tissues of the CHF rats, and the characteristic metabolites were finally derived by data processing with SPSS 19.0 software.Results The PAC and OPLS-DA models were established successfully.Ten characteristic metabolites with significant differences between the CHF and control groups, including lysophosphatidyl choline, lysophosphatidyl ethanolamine, oleic acid, glycocholic acid, and dehydroepiandrosterone sulfate, were screened and identified from the models.Conclusions The metabolic disorders in CHF rats are well fitted to the established metabolic profile models, and these identified characteristic metabolites may provide reference for the pathophysiological molecular mechanism and management, etc., of chronic heart failure.关键词
心力衰竭/色谱法/高压液相/质谱分析法/代谢组学/大鼠Key words
Chronic heart failure/Chromatography, high pressure liquid/Mass spectrometry/Metabolomics/Rat分类
医药卫生引用本文复制引用
巫菲,李彤,张磊,董志欢,米睿..慢性心力衰竭大鼠肝脏组织的代谢轮廓[J].中国比较医学杂志,2016,26(10):19-24,6.基金项目
天津市卫生局攻关项目（12KG106）。 （）
