中国比较医学杂志2016，Vol.26Issue(10)：32-35,68,5.DOI:10.3969.j.issn.1671-7856.2016.10.007
血管活性肠肽（VIP）对实验性自身免疫性脑脊髓炎（EAE）大鼠脑组织 IL-17A含量的影响
Effect of vasoactive intestinal peptide (VIP) on the content of IL-17A in the brain tissue of rats with experimental autoimmune encephalomyelitis (EAE)
杨元 1袁正洲 1吕志宇 1张淑江 1李晓红 1李作孝1
作者信息
- 1. 西南医科大学附属医院神经内科，四川泸州 646000
- 折叠
摘要
Abstract
Objective To explore the effect of vasoactive intestinal peptide (VIP) on the content of IL-17A in the brain tissue of rat models of experimental autoimmune encephalomyelitis ( EAE) .Methods Sixty healthy female Wistar rats were randomly divided into normal control group, EAE control group, low-dose VIP group and high-dose VIP group. Ten healthy guinea pigs were used to prepare anti-IL-17A antibody.Myelin basic protein ( MBP) +complete adjuvant ( CFA) were used to establish the EAE model.Since the first day of modelling, the low-dose and high-dose VIP groups received intraperitoneal injection of VIP 4 nmol/kg (0.2 mL) and 16 nmol/kg (0.8 mL), respectively, every other day for 10 consecutive days.The normal control group and EAE group were injected with 0.8 mL saline instead of VIP.The incubation period, progression and the peak of neurological dysfunction scores ( NDS) of the rats were recorded.The levels of IL-17A in the brain tissue was determined by ELISA assay, and the GFAP+astrocyte activation in brain at morbidity peak in the rats was examined using anti-GFAP ( glial fibrillary acidic protein) antibodies.Results The incubation period were extended, the progression period was shortened and the peak neuological dysfunction score ( NDS) was decreased in the VIP-treated groups, in a dose-response relationship.The cytokine levels of IL-17A and the astrocyte activation degree in brain tissue were reduced in each VIP dose group, in a dose-response relationship.Conclusions VIP exerts therapeutic effect on experimental autoimmune encephalomyelitis through lowering the IL-17A content and inhibition of astrocyte activation in the brain tissue.关键词
血管活性肠肽/实验性自身免疫性脑脊髓炎/IL-17A/GFAP+星型胶质细胞Key words
Vasoactive intestinal peptide/Experimental autoimmune encephalomyelitis/IL-17A/GFAP+astrocytes/Rats/Guinea pigs分类
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杨元,袁正洲,吕志宇,张淑江,李晓红,李作孝..血管活性肠肽（VIP）对实验性自身免疫性脑脊髓炎（EAE）大鼠脑组织 IL-17A含量的影响[J].中国比较医学杂志,2016,26(10):32-35,68,5.
