中国比较医学杂志2016，Vol.26Issue(10)：36-42,7.DOI:10.3969.j.issn.1671-7856.2016.10.008
槲芪散对肝癌前病变端粒酶活性的调控作用
Regulatory effect of a Chinese medicine prescription Hu Qi Shan on the telomerase activity in hepatic precancerous lesions in rats
摘要
Abstract
Objective To explore the possible mechanism of action of telomerase in hepatic precancerous lesions, and the regulatory effect of a Chinese medicine prescription HU Qi Shan ( HQS) and its principal drug mistletoe alkali on the telomerase activity in rat liver tissues.Methods Rat model of hepatic precancerous lesions was established by Solt-Farber two-step protocol.The model rats were randomly divided into 5 groups, including the model group, high-dose HQS [8 g/(kg· d)] group, low-dose HQS [4 g/(kg· d)] group, and mistletoe alkali[8 mg/(kg· d)] group.γ-Glutamy-transpeptidase (γ-GT) was analyzed by immunohistochemistry.AFP was detected by immunofluorescence technique.The telomerase activity was detected using a quantitative telomerase detection kit.The expression of NF-κB P65 was detected by immunohistochemistry.The cytoplasmic protein IκB-αwas detected by western blotting.Results After treated with HQS and mistletoe alkali, the areas ofγ-GT-positive foci and number of AFP-positive cells in the liver tissus were significantly decreased than those of the model group ( P<0.05 for both) , the telomerase activity was decreased, the number of NF-κB P65-positive cells was also decreased ( P <0.05 ) , whereas the intracytoplasmic expression of IκB-αproteins was significantly increased ( P <0.05 ) .Conclusions HQS and mistletoe alkali can suppress the telomerase activity.Its possible mechanism may be through inhibition of the over-expressed apoptosis-related genes such as NF-κB P65 and increase the expression of IκB-αdecreasing the telomerase activity.关键词
槲芪散/端粒酶/肝癌前病变/NF-ΚB P65/IκB-α/大鼠Key words
HQS/Telomerase/Hepatocarcinogenesis/NF-kB/IκB-α/Rat分类
医药卫生引用本文复制引用
孟霞,刘树红,李霞,丰平,卢静,王学江..槲芪散对肝癌前病变端粒酶活性的调控作用[J].中国比较医学杂志,2016,26(10):36-42,7.基金项目
国家自然科学基金资助项目（81272757）；北京市属高等学校创新团队建设与教师职业发展计划项目（ IDHT20150502）。 （）
