中国医学创新2016，Vol.13Issue(28)：1-6,6.DOI:10.3969/j.issn.1674-4985.2016.28.001
抑制AMPK激活对脑缺血小鼠行为学和脑梗死体积的影响
The Impact of Inhibiting Adenosine Monophosphate Activated Protein Kinase on the Behavior and Cerebral Infarction Volume of Mice after Cerebral Ischemia
摘要
Abstract
Objective:To discuss the impact of inhibiting adenosine monophosphate activated protein kinase(AMPK) on the behavior and cerebral infarction volume of mice after cerebral ischemia.Method:Sixty-six male Kunming mice were randomly divided into the sham operation group,saline control group and drug intervention group,each group had 22 mice.The AMPK specific inhibitor Compound C(20 mg/kg)was injected intraperitoneally in the drug intervention group during the time of ischemia,the middle cerebral artery occlusion/reperfusion model was made by thread embolism method,saline control group was given intraperitoneal injection of normal saline at the same time and the sham operation group did not give any drugs.After reperfusion for 24 h, the neurological function score was evaluated in mice,TTC staining was used to observe the volume of cerebral infarction and Westem-Blot method was used to detect the expression of pAMPK protein in the ischemic side of the brain.Result:There were no neurologic impairments and cerebral infarctions in the sham operation group, there was a small amount of pAMPK protein expression includes cortex(0.700±0.197) and hippocampus (0.690±0.228).After cerebral ischemia reperfusion injury,the neurological function score were(2.63±0.52) in the saline control group,the volume of cerebral infarction was(49.57±9.71)%,ischemia side brain tissue pAMPK protein includes the cortex(1.410±0.322) and the hippocampus (1.510±0.418),were higher than those of the sham operation group(P<0.05).The neurological function scores were(1.88±0.64)in the drug intervention group,the volume of cerebral infarction was(24.07±7.74)%,ischemia side brain tissue pAMPK protein includes the cortex(0.930±0.229) and the hippocampus (0.960±0.378),were lower than those of the saline control group(P<0.05).Conclusion:After cerebral ischemia reperfusion injury,AMPK is overactivated in ischemic brain,inhibiting AMPK plays neuroprotective effect.关键词
脑缺血再灌注损伤/磷酸腺苷活化蛋白激酶/神经保护Key words
Cerebral ischemia reperfusion injury/AMP-activated protein kinase/Neuroprotection引用本文复制引用
马毓,党辉,补娟,景燕,李红燕,朱沂..抑制AMPK激活对脑缺血小鼠行为学和脑梗死体积的影响[J].中国医学创新,2016,13(28):1-6,6.基金项目
新疆维吾尔自治区自然科学基金 （）
