中华耳科学杂志2016，Vol.14Issue(5)：670-673,4.DOI:10.3969/j.issn.1672-2922.2016.05.023
顺铂诱导斑马鱼侧线毛细胞损伤及再生模型的建立
Cisplatin-induced zebrafish lateral line hair cell loss and regeneration
芈肖肖 1严健 1李圆 2施军平1
作者信息
- 1. 杭州师范大学附属医院转化医学平台 杭州310015
- 2. 杭州师范大学附属医院耳鼻咽喉头颈外科 杭州310015
- 折叠
摘要
Abstract
Objective To establish a model of cisplatin-induced hair cell loss and regeneration in zebrafish. Methods Zebrafish larval were incubated in cisplatin solution. Using antibody staining, transgenic fish live imaging and in situ hybrid-ization, hair cell numbers before and after cisplatin treatment were characterized. Results Higher concentration cisplatin in-cubation caused more hair cell loss than lower concentration cisplatin when incubated for the same duration. Hair cells re-generation after lower concentration cisplatin incubation was also faster (P<0.001). Conclusion Cisplatin induced zebrafish lateral line hair cell loss and regeneration show dose and time-dependent patterns.关键词
顺铂/毛细胞损伤/毛细胞再生/剂量依赖效应Key words
Cisplatin/Hair cell injury/Hair cell regeneration/Dose-dependent manner分类
医药卫生引用本文复制引用
芈肖肖,严健,李圆,施军平..顺铂诱导斑马鱼侧线毛细胞损伤及再生模型的建立[J].中华耳科学杂志,2016,14(5):670-673,4.
