中华耳科学杂志2016，Vol.14Issue(5)：674-680,7.DOI:10.3969/j.issn.1672-2922.2016.05.024
反复发作性眩晕豚鼠前庭内侧核毒蕈碱受体M2、M3表达的研究
Expression of M2/M3 muscarinic receptors in medial vestibular nucleus in guinea pigs with recurrent vertigo
摘要
Abstract
Objective To study changes in expression of M2 and M3 muscarinic receptor subtypes in the medial ves-tibular nucleus using a guinea pig model of recurrent vertigo caused labyrinthine fistula. Method Adult guinea pigs (n=88) were randomly divided to receive sham-operation (group A, n=8), unilateral labyrinth lesioning (group B, n=40), or laby-rinthine fistula modeling and pneumatic otoscope treatment (group C, n=40). Guinea pigs in groups B and C were randomly selected to be sacrificed at 1 day, 3 days, 1 week, 2 weeks or 3 weeks post-operatively (n=8 at each time point), while ani-mals in group A were sacrificed at 3 weeks post-operation. Following fixative perfusion, the brain stem was harvested and expression of muscarinic receptors M2 and M3 subtypes in the MVN was examined by immunohistochemistry and com-pared among different groups. Results Muscarinic receptor M2/M3 subtypes decreased in MVN on both sides after opera-tion in animals from groups B and C. Expression was the lowest at day 1, and rose from day 3 to day 7 after operation. Sig-nificant difference was seen between groups A and C at day 7 after operation (P<0.05), but not between groups B and A. At 2 weeks after operation, there was no difference between groups C and A. M2/M3 expression in group C was also weaker than that in group B group before two weeks after operation). There was no side-to-side difference in any groups. Conclu-sion 1. M2 and M3 muscarinic receptor subtypes are expressed in the MVN in guinea pigs, with seemingly less M3 receptor expression than M2 receptor expression. These receptors may promote the normal process in the early stage of vestibular compensation. 2. In addition to the involvement of the cholinergic system, vestibular compensation may also involve other neurotransmitters or chemical substances. 3. The data provide additional evidence supporting the successful modeling of re-current vertigo by labyrinthine fistula.关键词
反复发作性眩晕/迷路瘘管/前庭代偿/前庭内侧核/毒蕈碱受体/豚鼠Key words
recurrent vertigo/labyrinthine fistula/vestibular compensation、medial vestibular nucleus/muscarinic re-ceptors/guinea pigs分类
医药卫生引用本文复制引用
饶秀丽,尹时华,侯涛,朱子昂,龙吉..反复发作性眩晕豚鼠前庭内侧核毒蕈碱受体M2、M3表达的研究[J].中华耳科学杂志,2016,14(5):674-680,7.基金项目
广西自然科学基金(基金任务书编号2015GXNSFAA414004) （基金任务书编号2015GXNSFAA414004）
广西自然科学基金（项目任务书编号2016GXNSFAA380150）Fund ProjectNatural Science Foundation of Guangxi （）
Natural Science Foundation of Guangxi （）
