肿瘤药学2016，Vol.6Issue(5)：343-348,6.DOI:10.3969/j.issn.2095-1264.2016.05.05
白花蛇舌草乙醇提取物对大肠癌耐药裸鼠移植瘤生长的影响
Effects of Ethanol Extract of HedyotisDiffusa Willd. on the Growth of Colorectal Cancer Drug-resistant Xenograft in Nude Mice
摘要
Abstract
ObjectiveTostudytheinhibitoryeffectofEthanolExtractofHedyotis diffusaWilld.(EEHDW)onthegrowthofcolorectal cancer drug-resistant xenograft in nude mice. Methods A drug-resistant subcutaneous xenograft model was established in nude mice. Mice were randomized into 4 groups, the Control, EEHDW, 5-FU, and EEHDW plus 5-FU combination groups. The inhibition rate was calculated by the determination of tumor volume. And the expressions of PCNA, Cyclin D1, CDK4 as well as p21 were detected by RT-PCR and IHC. Results Compared with the control group, 5-FU had little effect on the tumor growth (P>0.05), while EEHDW and combination treatment significantly inhibited the growth of xenograft (P<0.05), and combination treatment had more obvious inhibitory effect than that of EEHDW. EEHDW and combination treatment significantly inhibited the proliferation of tumor cells, down-regulated Cyclin D1 and CDK4, and promoted the mRNA and protein expression levels of p21 (P<0.05). Conclusion EEHDW could inhibit the growth of colorectal cancer drug-resistant xenograft. The mechanism might be related to its down-regulation of Cyclin D1 and CDK4 and up-regulation of p21 expressions.关键词
白花蛇舌草/大肠癌/5-氟尿嘧啶/肿瘤耐药/移植瘤Key words
Hedyotis diffusa Willd./Colorectal cancer/5-Fluorouracil/Drug resistance/Xenograft分类
医药卫生引用本文复制引用
靳祎祎,赖子君,严兆坤,冯健愉,彭军,林久茂..白花蛇舌草乙醇提取物对大肠癌耐药裸鼠移植瘤生长的影响[J].肿瘤药学,2016,6(5):343-348,6.基金项目
教育部博士点基金博导类（20133519110003），陈可冀中西医结合发展基金 （）
