现代农业科技Issue(21):115-115,117,2.
蝴蝶兰类原球茎分化的开放组培试验研究
Application of Open Culture in the Differentiation and Seedling of Phalaenopsis ssp. PLB
摘要
Abstract
Application of open culture with self developed bacteriostats in the differentiation and seedling of Phalaenopsis ssp. PLB was carried out.The culture medium was 1/2MS+3 g/L 6-BA+100 g/L mashed banana+20 g/L sucrose+1.0 g/L AC (activated charcoal)+6.3 g/L agar strips,and bacteriostats at reasonable concentration were added in medium of treatment.After culturing for 45 d,the results showed that bacteriostats could effectively inhibite microbial pollution,all PLB differentiated and formed plantlet.The average height of plantlet was(2.6±0.4)cm,which had no significant difference with that of the control(on autoclave sterilized medium).Blade number was 1~2 and the number of root lenghth above 0.5 cm was 1~3,both were consistent with that of the control.Therefore,it is really practical that the application of open culture in the differentiation and seedling of Phalaenopsis ssp. PLB.关键词
蝴蝶兰/开放组培/类原球茎/分化成苗Key words
Phalaenopsis ssp./open culture/PLB/differentiation and seedling分类
农业科技引用本文复制引用
刘福平,蔡晓东..蝴蝶兰类原球茎分化的开放组培试验研究[J].现代农业科技,2016,(21):115-115,117,2.基金项目
厦门科技计划项目(3502Z20162008)。 ()