安徽农业科学2016，Vol.44Issue(29)：144-146,3.
构树叶提取物体外抗病毒活性研究
Study on Antiviral Activity of Broussonetia papyrifera Leaf Extracts in vitro
摘要
Abstract
Objective] The present work was aimed to study the antiviral activity of Broussonetia papyrifera leaf extracts.[ Method] The MTT method was used to detect the effects of water, 75% ethanol and 50% acetone extracts of B.papyrifera leaf and different treatments on chick embryofibroblast( CEF) infected by newcastle disease virus ( NDV ) , infectious laryngotracheitis virus ( ILTV ) , infectious bronchitis virus (IBV) and infectious bursal disease virus(IBDV) respectively, to explore the antiviral activity and mechanism of B.papyrifera leaf extracts. [ Result] The result showed that the vitality of the NDV infected CEF was significantly increased owing to ethanol and acetone extracts supple-mented in the cell medium, the vitality of CEF infected by ILTV or IBV were also significantly improved by acetone extract addition, whereas the cytopathic effect induced by IBDV was not alerted by B.papyrifera leaf extracts.Moreover, the vitality of CEF pretreated by acetone ex-tract, followed by NDV or ILTV infection, tend to be higher than that of other treatments.[ Conclusion] It can be inferred that the effective components in the B.papyrifera extract may exert the antiviral effect by blocking virus recognition and adhesion to the host cells.关键词
构树叶/提取物/抗病毒活性Key words
Broussonetia papyrifera leaf/Extracts/Antiviral activity分类
农业科技引用本文复制引用
杜柏槐,刘晓军,陈绍红..构树叶提取物体外抗病毒活性研究[J].安徽农业科学,2016,44(29):144-146,3.基金项目
广东省农业厅科技计划项目（0809035）。 （）
