安徽医科大学学报2016，Vol.51Issue(12)：1753-1758,1759,7.DOI:10.19405/j.cnki.issn1000-1492.2016.12.008
雌激素治疗保护去卵巢对血管内皮细胞损伤的初步机制
Preliminary mechanism of estrogen therapy on ovariectomy-induced damages of vascular endothelial cells
摘要
Abstract
Objective To study the preliminary mechanism of estrogen therapy on estrogen deficiency-induced inju-ry of endothelium in thoracic aorta of female rats and human umbilical vein endothelial cells( HUVEC) . Methods 30 female SD rats were randomly divided into sham operation( SHAM) group,ovariectomized ( OVX) group, ovari-ectomized and estrogen treatment[ OVX+EST,0. 06 mg/( kg·d) ] group, and the treatment lasted for 6 months. HE and oil red O staining were used to observe the pathological changes of vascular endothelium. The blood vessel relaxation was analyzed by isolated vascular ring tension test. The changes of glucose regulated protein( GRP)78 and C/EBP homologous protein( CHOP) expression were examined by RT-PCR and Western blot respectively. HUVEC were divided into normal control ( CON ) group, normal +Tunicamycin control ( TM ) group, estrogen deficiency (ES-D) group, gradient concentrations of estrogen treatment(10 -9, 10 -8 and 10 -7 mol/L estradiol) group,and oxidative stress and endoplasmic reticulum stress indices were detected. Results The OVX rats showed greater weight,obvious injury and lipid depositionin in the endothelium of thoracic aorta. The OVX+EST rats showed im-proved above indices. In HUVEC cells, reactive oxygen species( ROS) were elevated and expressions of GRP78 and CHOP were increased in TM and ES-D group, different concentrations of estrodiol treatment alleviated the a-bove stress,and showed somewhat dose-dependent effect. Conclusion Estrogen deficiency cause damages of endo-thelium in thoracic aorta and HUVEC by increasing oxidative stress and endoplasmic reticulum stress, estrogen sup-plement inverts the above stress.关键词
雌激素缺乏/雌激素治疗/内质网应激/氧化应激/血管内皮Key words
estrogen deficiency/estrogen therapy/endoplasmic reticulum stress/oxidative stress/vascular endothe-lium分类
医药卫生引用本文复制引用
李朝飞,陈冠军,朱小欢,朱致祥,章秋,鲁云霞..雌激素治疗保护去卵巢对血管内皮细胞损伤的初步机制[J].安徽医科大学学报,2016,51(12):1753-1758,1759,7.基金项目
安徽省自然科学基金面上项目(编号:1308085MH154) （编号:1308085MH154）
