安徽医科大学学报2016，Vol.51Issue(12)：1790-1793,1794,5.DOI:10.19405/j.cnki.issn1000-1492.2016.12.016
淫羊藿次苷II通过p38 MAPK调控成骨细胞护骨素表达的体外研究
Effect of Icariside Ⅱ on the expression of osteoprotegerin in osteoblasts via p38 MAPK signaling pathway in vitro
摘要
Abstract
Objective To observe the effect of Icariside Ⅱ( ICA Ⅱ) on the expression of osteoprotegerin( OPG) and p38 mitogen-activated protein kinase(p38MAPK) in osteoblasts(OBs),explore the mechanism of ICAⅡther-apy in osteoporosis treatment. Methods Primary osteoblasts were cultured and randomly separated into four groups:control group, group ICA Ⅱ,mixed group(ICA Ⅱ+SB203580),group SB203580 (p38 signaling pathway inhibitor). ALP activity levels were detected by pNPP method. The protein levels of p38MAPK,p-p38MAPK and OPG were measured by Western blot. The expression of OPG mRNAs was detected by RT-PCR. Results Group ICA Ⅱ showed a significant increase in the level of ALP activity(P<0. 01),compared to the control group. ALP activity showed a decrease in the mixed group, compared to group ICAⅡ(P<0. 05). p-p38MAPK level in group ICA Ⅱ was remarkably higher than that of the control group ( P<0. 01 ) . The p-p38 MAPK level was significantly decreased in the mixed group (P<0. 01). Among all of the groups, the total p38MAPK protein level kept un-changed. The level of OPG in group ICAⅡwas remarkably higher than that of the control group (P<0. 01). The mixed group showed a decrease in the expression of OPG, compared to group ICAⅡ(P<0. 01). The gene expres-sion level of OPG was significantly upregulated in group ICAⅡcompared to the control group ( P<0. 01 ) . In the mixed group, the expression of OPG mRNA showed a decrease(P<0. 05). Conclusion ICAⅡcan stimulate the differentiation of osteoblasts, and promote the expression of OPG in osteoblasts. The process may be related to p38MAPK signaling pathway.关键词
淫羊藿次苷Ⅱ/成骨细胞/护骨素/p38MAPK/骨质疏松Key words
Icariside Ⅱ/osteoblasts/osteoprotegerin/p38 MAPK/osteoporosis分类
医药卫生引用本文复制引用
龚一昕,刘尚全,袁媛,张维娜..淫羊藿次苷II通过p38 MAPK调控成骨细胞护骨素表达的体外研究[J].安徽医科大学学报,2016,51(12):1790-1793,1794,5.基金项目
国家自然科学基金(编号:30840106) （编号:30840106）
