分析化学2016，Vol.44Issue(11)：1735-1741,7.DOI:10.11895/j.issn.0253-3820.160232
电喷雾质谱结合化学计量学方法快速筛选玛咖促睾丸间质细胞增殖活性成分
Rapid Screening of Active Components of Lepidium Meyenii Promoting Leydig Cell Proliferation by Electrospray Ionization Mass Spectrometry and Chemometrics Methods
摘要
Abstract
The low-polarity constituents from Lepidium meyenii in Yunnan and Peru were detected by electrospray ionization tandem mass spectrometry ( ESI-MS/MS ) . The effects of 10 samples of Lepidium meyenii on proliferation of rat leydig cells were measured. After the process of MS data using principal component analysis ( PCA ) methods, the samples from Yunnan and Peru were distinguished effectively. Subsequently, the grey relational degree analysis ( GRA ) and partial least squares analysis ( PLS ) were employed to reveal the correlation between common peaks of ESI fingerprint of Lepidium meyenii from different geographical origins and proliferation activity of rat leydig cells. The results suggested that most low-polarity constituents from Lepidium meyenii had good activities on proliferation of rat leydig cells, in which N-benzylhexadecanamide and N-benzy-(9Z,12Z,15Z)-octadecatrienamide exhibited the highest activities. In addition, the proposed procedure could also be used as a simple, quick reference method to screen the active components promoting Leydig cell proliferation in traditional Chinese medicine.关键词
玛咖/不同产地/电喷雾串联质谱/化学计量学方法/促睾丸间质细胞增殖Key words
Lepidium meyenii/Different geographical origin/Electrospray ionization tandem mass spectrometry/Chemometrics method/Promoting leydig cell proliferation引用本文复制引用
孙佳明,田淋淋,何忠梅,孟令文,孙慧,阚俊明,张辉..电喷雾质谱结合化学计量学方法快速筛选玛咖促睾丸间质细胞增殖活性成分[J].分析化学,2016,44(11):1735-1741,7.基金项目
本文系国家自然科学基金(No.31570347),吉林省卫生和计划生育委员会科研计划课题(No.2015Z066)资助 （No.31570347）
