高校化学工程学报2016，Vol.30Issue(5)：1134-1139,6.DOI:10.3969/j.issn.1003-9015.2016.05.021
无溶剂体系丙三醇改性硅烷凝胶及应用于固定化脂肪酶
Solvent-Free Glycerin Modified Tetraethoxysilane Sol-Gel and its Application in Lipase Immobilization
钱俊青 1唐弋奥 1李尚谦1
作者信息
- 1. 浙江工业大学 药学院，浙江 杭州 310014
- 折叠
摘要
Abstract
Solvent-free glycerin modified tetraethoxysilane (TEOS) sol-gel was prepared to obtain enzyme immobilization materials without toxic reagents and suitable for food industry. The material prepared has good food safety and high biocompatibility, and the optimum modification conditions is TEOS:glycerin molar ratio=1:0.25, solid acid catalyst 3 g.(50 mL)-1 TEOS, temperature 50℃ for 72 h. The hydrophilicity of the modified TEOS is significantly increased with water solubility of 0.58 g . mL-1. The lipase immobilized shows high catalytic activity and strong structural rigidity. L-ascorbyl palmitate synthesis catalyzed by immobilized and free enzymes is studied under same conditions, and the immobilized enzyme shows better performance.关键词
丙三醇改性/硅烷凝胶/无溶剂体系/固定化/脂肪酶Key words
glycerin modifying/sol-gel/solvent-free system/immobilization/lipase分类
生物科学引用本文复制引用
钱俊青,唐弋奥,李尚谦..无溶剂体系丙三醇改性硅烷凝胶及应用于固定化脂肪酶[J].高校化学工程学报,2016,30(5):1134-1139,6.
