华南农业大学学报2016，Vol.37Issue(6)：52-58,7.DOI:10.7671/j.issn.1001-411X.2016.06.008
猪生殖细胞特异性基因 Sohlh1及启动子的研究
Analysis of pig germ-cell special gene Sohlh1 and its promoter
摘要
Abstract
Objective] To study the expression of germ-cell special gene Sohlh1 in different tissues of young and adult pigs, analyze the features of molecular genetic evolution of Sohlh1 gene, and determine the core region and specific regulatory region of the promoter.[Method]The expressions of Sohlh1 gene in different pig tissues and different time were verified by RT-PCR.The features of molecular genetic evo-lution of Sohlh1 gene was analyzed using biology software, the promoter was constructed and confirmed by a verification vector, and the core region and specific regulatory region of the promoter were analyzed by dual luciferase assay.[Result]Sohlh1 gene was expressed in testis of piglet and adult pig and ovary of piglet, and was not expressed in ovary of adult pig.Sohlh1 gene had evolved along with the evolution of lower to higher vertebrates based on phylogenetic analysis of SOHLH1 proteins in multiple species.The core region of the promoter of pig Sohlh1 gene was near 78 bp, and the specific regulatory region was be-tween 771 and 2 981 bp based on dual luciferase assay of the promoter verification vector.[Conclusion]Pig could be a superior animal model compared with rodent based on Sohlh1 gene evolution analysis.The tissue specific expression of Sohlh1 gene may be mainly controlled by the specific regulatory region of its promoter.关键词
猪/Sohlh1/启动子/遗传进化/验证载体/双荧光素酶Key words
pig/Sohlh1/promoter/genetic evolution/verification vector/dual luciferase分类
农业科技引用本文复制引用
刘凯,刘国乾,曹亚鸽,卫恒习,李莉,张守全..猪生殖细胞特异性基因 Sohlh1及启动子的研究[J].华南农业大学学报,2016,37(6):52-58,7.基金项目
973计划项目（2011CB944202）；国家自然科学基金 （）
