华南农业大学学报2016，Vol.37Issue(6)：65-69,5.DOI:10.7671/j.issn.1001-411X.2016.06.010
食线虫性真菌弯孢节丛孢菌的分离及捕食线虫过程的观察
Isolation of nematophagous fungus Arthrobotrys musiformis and observation of nematode predating process
摘要
Abstract
[Objective] To isolate nematophagous fungus Arthrobotrys musiformis from substances associat-ed with cattle and sheep, and observe the process of the fungus predating third-stage larvae of Haemon-chus contortus and free-living nematode Caenorhabditis elegans.[Method]A.musiformis was isolated using baited plate technique, and the dynamic processes of A.musiformis predating H.contortus and C. elegans were observed under scanning electron microscopy ( SEM ) .A.musiformis isolate NPS045 was inoculated onto 20 g· L-1 water agar plate covered with cellulose membrane on agar surface, incubated for four days, and then the nematodes were added to the plates.The samples containing nematodes and fungi were collected and observed at different time points after the two nematode species were captured.[Result]SEM analysis showed that 5 hours after the two nematode species were added, A.musiformis produced predating structures and started to capture the nematodes.The third-stage larvae of H.contortus were penetrated by A.musiformis 22 hours after being caught, invasive hyphae grew out of the larval body after 56 hours, and the larvae were completely digested by 68 hours.C.elegans were penetrated by A.musiformis and clearly shrunk 14 h after being caught, seriously collapsed after 19 hours, and were completely digested by the 24th hour.[Conclusion]Seventeen A.musiformis strains were isolated from 1 502 samples associated with cattle and sheep, and the prevalence of A.musiformis in total samples was 1.13%.关键词
弯孢节丛孢菌/捻转血矛线虫/秀丽隐杆线虫/生物防治Key words
Arthrobotrys musiformis/Haemonchus contortus/Caenorhabditis elegans/biological control分类
农业科技引用本文复制引用
王逢会,王波波,蔡葵蒸..食线虫性真菌弯孢节丛孢菌的分离及捕食线虫过程的观察[J].华南农业大学学报,2016,37(6):65-69,5.基金项目
教育部长江学者和创新团队发展计划 （）
