华南农业大学学报2016,Vol.37Issue(6):70-76,7.DOI:10.7671/j.issn.1001-411X.2016.06.011
人工诱导白木香树体抗逆能力的研究
Study on resistance ability of Aquilaria sinensis trees under artificial induction
摘要
Abstract
[Objective] To study the effects of hormone, salt, fungi and artificial wounding induction on resistance ability of Aquilaria sinensis trees, and discover the mechanism of agarwood formation in A. sinensis trees.[Method]We conducted the experiment in Huidong county, Guangdong province, and used a random block design with three single tree repeats per treatment.Six months after stimulation, stress-resistance related substances and enzyme activities were analyzed for A.sinensis tree samples.[Result]Among the different treatments of hormone, salt, fungi and artificial wounding, the optimal treatments were treatment 2 (MeJA and Et), treatment 8 [NaHSO3(w=1‰), NaCl(w=20‰) and FeCl2(w=10‰) ] , treatment 10 ( Trichoderma atroviride ) and treatment 16 ( burning-chisel-drilling ) .For these four treatments, the contents of essential oil were 8.48%, 9.86%, 12.46% and 6.21% respectively, approaching or reaching agarwood quality standard ( 10%) .Polyphenol, soluble sugar content, and CAT, POD, SOD, NOS, PAL activities were higher compared to other treatments of the same treatment type, while MDA and starch contents were relatively lower.Based on principal component analysis, the scores of resistance ability of A.sinensis trees with treatment 2, 8, 10 and 16 were 1.83,2.31,2.10 and-1.51, respectively, all highest among the same treatment type.[Conclusion] MeJA and Et, NaHSO3 ( w=1‰) , NaCl ( w=20‰) and FeCl2 ( w=10‰) , Trichoderma atroviride and burning-chisel-drilling could induce strong resistance, and could promote agarwood formation of A.sinensis trees.关键词
白木香/人工诱导/抗逆/结香Key words
Aquilaria sinensis/artificial induction/stress resistance/agarwood formation分类
农业科技引用本文复制引用
王东光,张宁南,杨曾奖,刘小金,洪舟,徐大平..人工诱导白木香树体抗逆能力的研究[J].华南农业大学学报,2016,37(6):70-76,7.基金项目
国家林业局林业公益性行业科研专项(201304402-4);广东省林业科技创新项目 ()