临床口腔医学杂志2016，Vol.32Issue(11)：646-649,4.DOI:10.3969/j.issn.1003-1634.2016.11.002
微纳复合结构对成纤维细胞黏附增殖的影响
Effects of micro/nano structured surfaces coatings on fibroblasts adhesion and proliferation interactions with ti-tanium dental implants
摘要
Abstract
Objective:To evaluate fibroblast attachment and proliferation on different types titanium surfaces.Meth-ods:There were 36 test specimens were assigned to 3 group that with pure titanium surfaces treated by machined of group A, sand blasting and acid etching of group B,and sand blasting and acid-alkali heat of group C.The surface topography of three groups were characterized by using a field emission scanning electron microscope( FESEM) .With an confocal laser scanning microscope( CLSM) ,the surface roughness was analyzed.Mouse fibroblastic cells L929 cultured on the discs were evaluated in terms of cellular adhesion and proliferation by CCK-8 kit at the wavelength of 450 nm.Results:The test specimens surface of group A were smooth,and fibroblasts exhibited spindle shapes with small expansion.While those were rough of group B and C,which cells grown on were small but featured an angular or circular shape.In addtion,a hierarchical structure of a micro/nano porous surface was constructed on group C.In attachment activity,the cell number was higher of group C than group A or B.The rough surface was better than the smooth surface at the day of 1,3 and 5 in proliferative activity.Conclusion:The micro/nanotopographic surfaces created by sand blasting and acid-alkali heat enhanced cellar adhesion,and was no harm to proliferation.Pure titanium decorated with micro/nano porous provided a more compatible surfaces for fibroblasts to live with, and may provide a new design for the implant.关键词
表面处理/微纳米复合结构/成纤维细胞/种植体Key words
Surface treatment/Micro/nano structure/Fibroblasts/Implant分类
医药卫生引用本文复制引用
赵颖,韩石磊,李大鹏,谢佳雨,刘滋川,杜青,谢伟丽..微纳复合结构对成纤维细胞黏附增殖的影响[J].临床口腔医学杂志,2016,32(11):646-649,4.基金项目
黑龙江省科技攻关项目 （）
