临床口腔医学杂志2016，Vol.32Issue(11)：650-653,4.DOI:10.3969/j.issn.1003-1634.2016.11.003
基于 NF-κB 信号通路倍半萜烯内脂化合物对牙周病破骨细胞活化因子作用的研究
Study on the effect of sesquiterpene lactone compounds on osteoclast activating factor IL-1 in periodontal dis-ease based on NF-κB signaling pathway
摘要
Abstract
Objective:To study the effect of sesquiterpene lactone compounds on osteoclast activating factor IL-1 in hPDLF periodontal disease based on NF-κB signaling pathway.Method:Fifteen 2-weeks-old rats with periodontal disease were selected as the observation group,and 6 healthy 2-weeks-old rats were selected as the control group.The observation group's rats were randomly divided into 3 groups.The rats were injected 2.5 mg/kg/d parthenolide(PAR)in group 1,injected 2 mg/kg/d salvia roborowskii active monomer in group 2,and injected daily with the same amount of saline in group 3 and the control group.Osteoclast activating factor IL-1 mRNA and protein expression were measured by fluorescence quantitative polymerase chain reaction ( PCR ) and enzyme linked immune assay ( ELISA ) method of periodontal ligament fibroblasts ( PDLF) based on NF-κB signaling pathway in periodontal disease.And the treatment of periodontal disease in the observation group was observed.Results:Compared with the control group(1.02 ±0.08,3.2 ±0.1)ng,there were no statistically signifi-cant difference in the NF-κB and IL-1 content of rats periodontal tissue both in group 1(1.1 ±0.11,3.4 ±0.15)ng and group 2(1.12 ±0.05,3.36 ±0.13)ng,respectively(P>0.05).The content of NF-κB and IL-1 in group 3(3.92 ±0.03,8. 59 ±0.16)ng when compared with group 1 and group 2 respectively,there were statistically significant differences(P<0. 05).After 2 weeks of the treatment by sesquiterpene lactone compounds including parthenolide and salvia roborowskii active monomer both in group 1 and group 2,the periodontal conditions have been improved.Conclusion:The treatment effect of sesquiterpene lactone compounds on osteoclast activating factor IL-1 in periodontal disease based on NF-κB signaling pathway have been proved.关键词
NF-κB信号通路/小白菊内酯/粘毛鼠尾草/牙周膜成纤维细胞/牙周病/破骨细胞活化因子Key words
NF-κB signaling pathway/Parthenolide/Salvia roborowskii/Periodontal ligament fibroblasts/Periodontal disease/Osteoclast activating factor分类
医药卫生引用本文复制引用
詹春华,刘冰,黄杏颜,翁春辉..基于 NF-κB 信号通路倍半萜烯内脂化合物对牙周病破骨细胞活化因子作用的研究[J].临床口腔医学杂志,2016,32(11):650-653,4.基金项目
广东省科技厅引导项目 （）
