农业环境科学学报2016，Vol.35Issue(11)：2163-2170,8.DOI:10.11654/jaes.2016-0506
生活污水对土壤及湿地植物根际细菌群落的影响
Impacts of domestic sewage on community structure and diversity of bacteria in the soil and rhizospheres of five wetland plants
摘要
Abstract
The structure and diversity of bacterial communities were studied within the soil and within the rhizospheres of five wetland plants treated with tap water and sewage water using the PCR-DGGE technique. Results indicate that the dominant bacterial communities undergo a shift both within bare soil, and within the wetland plant root zone with the addition of sewage water, The bacterial community diversity in-dex was highest within the rhizospheres of wetland plants that were treated with sewage water, followed by the soil without plants that was treated with sewage water, and then within the root zone of wetland plants immersed in tap water. The bacterial community diversity index was lowest in the un-amended experimental soil used for plant culture. The dominant phyla of the bacterial communities were analyzed by denaturing gradient gel electrophoresis(DGGE)and sequencing of partial 16S rRNA genes. There were eight genera of bacteria detected in our study overall. Within the un-amended experimental soil treatment, Rhizobium and Sphingomonas were the dominant genera; in the treatment with just soil and sewage, Sphingomonas, Rhizobium, Arthrobacter and Erythrobacter were the dominant genera; within the rootzones of the wetland plants in tap water, Flavobacterium and Arthrobacter were the dominant genera;and within the root zones of the wet-land plants in sewage water, Arthrobacter, Clostridium, Sulfate-reducing bacteria, Proteiniclasticum and Flavobacterium were dominant. This study provides evidence that sewage may promote the growth of diverse microbial communities in soil and within the rhizospheres of wetland plants.关键词
湿地植物/PCR-DGGE/土壤微生物/多样性Key words
wetland plants/PCR-DGGE/edaphon/diversity分类
农业科技引用本文复制引用
李龙山,倪细炉,李昌晓,李健..生活污水对土壤及湿地植物根际细菌群落的影响[J].农业环境科学学报,2016,35(11):2163-2170,8.基金项目
国家国际科技合作专项项目(2015DFA90900，2011DFG32780）；国家自然科学基金项目 （）
