山东科学2016，Vol.29Issue(5)：54-59,75,7.DOI:10.3976/j.issn.1002-4026.2016.05.009
铜、汞和铅对斑马鱼仔鱼肝脏发育的影响
lmpact of Cu,Hg and Pb on liver growth of zebrafish larvae
摘要
Abstract
We addressed the impact of three typical heavy metal pol utants ( copper chloride, mercury chloride and lead acetate) on liver growth of zebrafish larvae (72~144 hpf) with a liver green fluorescence transgenic zebrafish T3 (lfabp:EGFP) as a model. Results show that the survival rates of al groups are al more than 95%. CuCl2 and HgCl2 of 1 μmol/L can al cause abnormality of swimbladder growth or inflation. The three heavy metal compounds can al significantly reduce liver fluorescence area and integral optical density. CuCl2 has more powerful inhibition capability for liver growth than Pb( Ac) 2 and HgCl2 under identical exposure concentration. However, HgCl2 of 0. 01 μmol/L can inhibit liver growth. lnhibition capability of lfabp expression of CuCl2 and Pb( Ac) 2 is greater that of HgCl2 . Moreover, Pb( Ac) 2 of 0.01μmol/L can inhibit the expression of lfabp. Results show that the three heavy metal compounds can al significantly inhibit liver growth. Transgenic zebrafish larvae can serve as priority model for detection of environmental contaminants caused liver growth toxicity.关键词
铜/汞/铅/斑马鱼/仔鱼/肝脏发育Key words
copper/mercury/lead/zebrafish/larvae/liver growth分类
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韩建,彭维兵,韩利文,何秋霞,张云,孙晨,王荣春,楚杰,刘可春..铜、汞和铅对斑马鱼仔鱼肝脏发育的影响[J].山东科学,2016,29(5):54-59,75,7.基金项目
山东省科学院青年基金(2015QN012) （2015QN012）
山东省自主创新及成果转化专项(2014ZZCX02105) （2014ZZCX02105）
山东省自然科学基金三院联合基金( ZR2015YL013) （ ZR2015YL013）
