山东医药2016，Vol.56Issue(39)：1-4,4.DOI:10.3969/j.issn.1002-266X.2016.39.001
耐药人胃癌细胞U HRF 1基因的表达变化及其与多药耐药的关系
Expression of UHRF1 gene in gastric cancer resistant cells and its relationship with multidrug resistance
摘要
Abstract
Objective To observe the expression changes of ubiquitin-like with PHD and ring finger domain 1 (UHRF1 )in gastric cancer drug-resistance cells,and to explore the relationship with gastric cancer multidrug resistance. Methods We examined UHRF1 mRNA and protein expression in GC multidrug-resistant cell lines SGC7901/VCR and SGC7901/ADR and their parental cell line SGC7901 by RT-PCR and Western blotting.Small interfering RNA (siUHRF1) was transfected into SGC7901/VCR and SGC7901/ADR cells (SGC7901/VCR-siUHRF1,SGC7901/ADR-siUHRF1),re-spectively;and blank control group without any treatment was set.UHRF1 plasmid with overexpression (SGC7901-UHRF1)and empty blank plasmid (SGC7901-NC)were transfected into SGC7901 cells,and blank control group was also established.After 48-hour transfection,the cells were collected for further use.MTT assay was conducted to detect the GC cell line sensitivity to several chemotherapy drugs,including VCR,ADR,5-FU and CDDP,and IC50 was calculated.5-FU was co-cultured with gastric cancer cell lines and the apoptosis was observed by flow cytometry (FCM).Results The rela-tive mRNA and protein expression of UHRF1 in SGC7901 cell line was down-regulated as compared with that of SGC7901/VCR and SGC7901/ADR cells (all P<0.05 ).Promoting the expression of UHRF1 could increase the IC50 of SGC7901 cells to VCR,ADR,5-FU and CDDP and the apoptosis was decreased (all P<0.05).Silencing UHRF1 expression could reduce the IC50 of SGC7901/VCR and SGC7901/ADR cells to VCR,ADR,5-FU and CDDP,and the apoptosis rate was increased (all P<0.05).Conclusions UHRF1 is highly expressed in gastric cancer drug-resistance cell lines,and the high expression of UHRF1 may decrease drug sensitivity and improve multidrug resistance of gastric cancer,which may be related to the inhibition of UHRF1 on the apoptosis of gastric cancer cells.关键词
胃癌/泛素样含PHD和环指域1/多药耐药/药物敏感性/半数抑制浓度/细胞凋亡Key words
gastric carcinoma/ubiquitin-like with PHD and ring finger domain 1/multidrug resistance/drug sensi-tivity/median inhibitory concentration/apoptosis分类
医药卫生引用本文复制引用
周林,李伟,韩东升,张伟..耐药人胃癌细胞U HRF 1基因的表达变化及其与多药耐药的关系[J].山东医药,2016,56(39):1-4,4.基金项目
国家自然科学基金资助项目（81402337）。 （）
