山东医药2016，Vol.56Issue(39)：12-15,4.DOI:10.3969/j.issn.1002-266X.2016.39.004
1，25-二羟基维生素D3对人肾小球系膜细胞增殖、周期的影响及其机制
Effects of 1,25(OH)2 D3 on proliferation and cell cycle of human glomerular mesangial cells
摘要
Abstract
Objective To investigate the effects of 1 ,25-dihydroxyvitamin D3 [1 ,25 (OH)2 D3 ]on cell proliferation and cell cycle of human glomerular mesangial cells and the possible mechanism.Methods Human mesangial cells in the logarithmic phase were cultured in vitro and then were divided into four groups:groups A,B,C and D.Four groups were separately added with such culture mediums:group A with 10 -8 mol/L 1,25(OH)2D3 +L-DMEMmedium containing 5%fetal bovine serum (FBS),group B with 5 μg/mL rapamycin +L-DMEM medium containing 5% FBS,group C with 5μg/mL rapamycin +10 -8 mol/L 1,25(OH)2D3 +L-DMEMmedium containing 5%FBS,and group D with the L-DMEM medium containing 5% FBS.After 48-hour administration,we observed the cell proliferation and cell cycle distribution in each group.The protein expression of eukaryotic cell translation initiation inhibitory factor 4E binding protein (4E-BP1 ) and phosphorylated 4E-BP1 (P-4EBP1 )in cells was determined by Western blotting.Results At 48 h,the cell apoptosis rates of the groups A,B and C were 38.02%,55.62% and 99.23%,respectively,and significant difference was found between them (P<0.05).At 48 h,the proportion of cells in the G1 phase of groups A,B and C was significantly higher than that of group D (all P<0.05),the proportion of cells in the S,G2/Mphase was clearly lower than that of group D (all P<0.05).The proportion of cells in the G1 phase of groups B and C was significantly higher than that of group A (all P<0.05),and the proportion of cells in the S,G2/Mphase was clearly lower than that of group D (all P<0.05).The proportion of cells in the G1 phase of group C was higher than that of group B (all P<0.05),and the proportion of cells in the S,G2/Mphase was lower than that of group B (all P<0.05).The 4E-BP1 and P-4E-BP1 protein expression and P-4E-BP1/4E-BP1 of groups A,B and C was lower than that of group D (all P<0.05).The 4E-BP1 and P-4E-BP1 protein expression and P-4E-BP1/4E-BP1 of groups B and C was lower than that of group A (all P<0.05).The 4E-BP1 and P-4E-BP1 protein expression and P-4E-BP1/4E-BP1 of group C was lower than that of group B (all P<0.05).Conclusion 1 ,25 (OH)2 D3 may inhibit the glomerular mesangial cell proliferation and block the cell cycle in G1 phase through down-regulating the expression of 4E-BP1 .关键词
人肾小球系膜细胞株/1 ,25-二羟维生素D3/真核细胞翻译起始抑制因子4E结合蛋白/细胞增殖/细胞周期Key words
human glomerular mesangial cell line/1 ,25-dihydroxyvitamin D3/eukaryotic cell translation initiation inhibitory factor 4E binding protein/cell proliferation/cell cycle分类
医药卫生引用本文复制引用
聂晴晴,唐玉玲,赵丹,张春江,杨晓萍..1，25-二羟基维生素D3对人肾小球系膜细胞增殖、周期的影响及其机制[J].山东医药,2016,56(39):12-15,4.基金项目
国家自然科学基金资助项目（81160090）。 （）
