山东医药2016，Vol.56Issue(42)：1-4,4.DOI:10.3969/j.issn.1002-266X.2016.42.001
二甲双胍对脂多糖激活小胶质细胞炎症因子释放的影响及机制探讨
Effect of metformin on release of inflammatory factor from LPS-stimulated microglia and its possible mechanism
摘要
Abstract
Objective To observe the effect of metformin on the release of inflammatory factor from lipopolysaccharide (LPS)-stimulated microglia and to investigate the possible mechanism.Methods The cytotoxicity of metformin to BV2 cells in logarithmic growth phase was tested by Trypan blue exclusion.Then BV2 cells were randomly divided into LPS stimulation group (LPS group,cells were cultured with LPS in 50 ng/mL for 24 hours)and LPS plus Metformin group (ob-servation group,cells were cultured with metformin in 0.02,2,8,and 16 mmol/L for 24 hours first,then cultured with LPS in 50 ng/mL for 24 hours).TNF-αwas detected by ELISA.The proliferation was calculated by Cell counting.The cell viability was tested by MTT.The cell cycle was evaluated by propidium iodide staining +flow cytometry.The apoptot-ic rate was analyzed by improved propidium iodide staining +flow cytometry.Results The microglia treated with differ-ent concentrations of metformin refused staining of the trypan blue exclusion.Compared with LPS group,metformin de-creased the level of TNF-α,inhibited the proliferation of microglia and increased the apoptotic rate in a dose-dependent manner (P <0.05,0.01,0.01 at 2,8,and 16 mmol/L respectively).With the increased metformin concentration,the percentage of microglia in the G1 phase was increased.Significant difference was found in the percentage of microglia in the G1 phase between the LPS group and observation group with 8 and 16 mmol/L (P <0.05,P <0.01).Conclusion Met-formin decreases the release of inflammatory factor from LPS-stimulated microglia,and the mechanism may be related to the inhibition of microglia proliferation and promotion of microglia apoptosis.关键词
二甲双胍/小胶质细胞/脂多糖/肿瘤坏死因子/细胞周期/细胞凋亡Key words
metformin/microglia/lipopolysaccharide/tumor necrosis factor/cell cycle/apoptosis分类
医药卫生引用本文复制引用
王海燕,张俊峰,郭勇,贾佩玉,王学敏..二甲双胍对脂多糖激活小胶质细胞炎症因子释放的影响及机制探讨[J].山东医药,2016,56(42):1-4,4.基金项目
国家自然科学基金资助面上项目（81571035）。 （）
